Rabbit Anti-NUDT5 Recombinant Antibody (clone R65-3J-2)
CAT#: VS3-FY2140
This R65-3J-2 recombinant monoclonal antibody exhibits specific targeting for human NUDT5 protein, a calculated weight of 24 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R65-3J-2 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19827/if_selected.jpg
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NUDT5
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R65-3J-2
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 24 kDa, while the observed molecular weight stands at 30kDa.
- Related Disease
- Metabolism of nucleotides and Metabolism
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Target
- Alternative Names
- Nudix Hydrolase 5; Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 5; Nuclear ATP-Synthesis Protein NUDIX5; 8-Oxo-DGDP Phosphatase; HNUDT5; YSA1H; Nucleoside Diphosphate-Linked Moiety X Motif 5; ADP-Sugar Pyrophosphatase
- Gene ID
- 11164
- UniProt ID
- Q9UKK9
- Sequence Similarities
- Belongs to the Nudix hydrolase family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylation at Thr-45 is required for homodimer stability; dephosphorylation results in destabilization of the homodimer. Dephosphorylation at Thr-45 promotes the ATP-synthesis activity.
- Protein Refseq
- NP_054861.2
- Function
- Enzyme that can either act as an ADP-sugar pyrophosphatase in absence of diphosphate or catalyze the synthesis of ATP in presence of diphosphate.
In absence of diphosphate, hydrolyzes with similar activities various modified nucleoside diphosphates such as ADP-ribose, ADP-mannose, ADP-glucose, 8-oxo-GDP and 8-oxo-dGDP.
Can also hydrolyze other nucleotide sugars with low activity.
In presence of diphosphate, mediates the synthesis of ATP in the nucleus by catalyzing the conversion of ADP-ribose to ATP and ribose 5-phosphate. Nuclear ATP synthesis takes place when dephosphorylated at Thr-45.
Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming.
Does not play a role in U8 snoRNA decapping activity (By similarity).
Binds U8 snoRNA (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NUDT5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2209MZ | Recombinant Mouse Anti-Human NUDT5 Antibody | WB | Mouse antibody |
| VS4-CJ62 | Recombinant Rabbit Anti-NUDT5 Antibody (clone 10D2) | ELISA, WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2516 | Hi-Affi™ Recombinant Rabbit Anti-NUDT5 Monoclonal Antibody (DS2516AB) | WB, IHC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC637 | AbPlus™ Anti-NUDT5 Magnetic Beads (VS-0724-YC637) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5040 | Anti-NUDT5 Immunohistochemistry Kit | IHC |
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