Rabbit Anti-NUDT5 Recombinant Antibody (clone R65-3J-2) (CAT#: VS3-FY2140)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat NUDT5

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R65-3J-2

• Applications
• Western Blot, Immunohistochemistry-Paraffin

• Conjugate
• Unconjugated

• MW
• The calculated weight of the target protein is approximately 24 kDa, while the observed molecular weight stands at 30kDa.

• Related Disease
• Metabolism of nucleotides and Metabolism

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS (pH 7.4), 150 mM NaCl, 50% glycerol

• Preservative
• 0.02% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/100-1/200

• Alternative Names
• Nudix Hydrolase 5; Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 5; Nuclear ATP-Synthesis Protein NUDIX5; 8-Oxo-DGDP Phosphatase; HNUDT5; YSA1H; Nucleoside Diphosphate-Linked Moiety X Motif 5; ADP-Sugar Pyrophosphatase

• Gene ID
• 11164

• UniProt ID
• Q9UKK9

• Sequence Similarities
• Belongs to the Nudix hydrolase family.

• Cellular Localization
• Nucleus

• Post Translation Modifications
• Phosphorylation at Thr-45 is required for homodimer stability; dephosphorylation results in destabilization of the homodimer. Dephosphorylation at Thr-45 promotes the ATP-synthesis activity.

• Protein Refseq
• NP_054861.2

• Function
• Enzyme that can either act as an ADP-sugar pyrophosphatase in absence of diphosphate or catalyze the synthesis of ATP in presence of diphosphate.
  In absence of diphosphate, hydrolyzes with similar activities various modified nucleoside diphosphates such as ADP-ribose, ADP-mannose, ADP-glucose, 8-oxo-GDP and 8-oxo-dGDP.
  Can also hydrolyze other nucleotide sugars with low activity.
  In presence of diphosphate, mediates the synthesis of ATP in the nucleus by catalyzing the conversion of ADP-ribose to ATP and ribose 5-phosphate. Nuclear ATP synthesis takes place when dephosphorylated at Thr-45.
  Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming.
  Does not play a role in U8 snoRNA decapping activity (By similarity).
  Binds U8 snoRNA (By similarity).

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

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