Rabbit Anti-NUP153 Recombinant Antibody (clone 8D4) (CAT#: ZG-0491U)
Rabbit Anti-NUP153 Antibody (clone 8D4) displayed high specificity against the NUP153 protein of Human.
Specific Inquiry
IHC
Figure 1 Rabbit Anti-NUP153 Antibody (ZG-0491U) in IHC
IHC image of ZG-0491U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IF
Figure 2 Rabbit Anti-NUP153 Antibody (ZG-0491U) in IF
Immunofluorescence staining of Hela Cells with ZG-0491U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human Nup153
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NUP153
- Species Reactivity
- Human
- Clone
- 8D4
- Applications
- ELISA, IHC, IF
- Conjugate
- Unconjugated
- Related Disease
- Carnitine-Acylcarnitine Translocase Deficiency and 3-Methylglutaconic Aciduria, Type Iv.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Immunofluorescence. Specific conditions should be optimized by the end user.
IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- Nuclear pore complex protein Nup153; 153 kDa nucleoporin; Nucleoporin Nup153; NUP153
- Gene ID
- 9972
- UniProt ID
- P49790
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NUP153"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2351CT | Recombinant Mouse anti-Human NUP153 Monoclonal antibody (nup7A8) | IHC-Fr, WB, IP | |
| MRO-1120-CN | Recombinant Rabbit Anti-NUP153 Monoclonal Antibody (JU40-41) | WB, IF, FC | Rabbit IgG |
| VS1-YJ156 | Sheep Anti-NUP153 Antibody (VS-0722-YJ156) | IB, IF, IP | Sheep IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for ZG-0491U. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, WB
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA
Application: Neut, Inhib, FC, ELISA
Application: WB, ELISA, IHC, IP, SPR, FuncS
Application: FC
Application: Neut, FuncS, IHC, ELISA, FC, Inhib
Application: ELISA, Block, WB, FC, IP
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
