CytoStream™ Rabbit Anti-OCLN Polyclonal Antibody (VS13-YC853)
CAT#: VS13-YC853
The CytoStream™ Rabbit Anti-OCLN Polyclonal Antibody is specific for human, mouse and rat OCLN. OCLN encodes a tight junction protein regulating paracellular permeability, with mutations linked to neurological disorders like BLC-PMG. The anti-OCLN antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1 μg per 1x 10⁶ cells).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5933/if_selected.jpg
Normal Tissue
Figure 2 Thyroid gland
High expression in thyroid gland
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5933/15984_B_1_5_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32513_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32314_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32314_A_7_4.jpg
Normal Tissue
Figure 6 Gallbladder
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32314_A_6_4.jpg
Normal Tissue
Figure 7 Kidney
Collecting ducts Staining: Medium Intensity: Moderate Quantity:>75% Distal tubules Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32314_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32314_A_6_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (A-431, CAPAN-2, HaCaT, RPTEC TERT1, SK-BR-3)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000197822-OCLN
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Specifications
- Immunogen
- Recombinant human Occludin, aa 151-250, conjugated with KLH.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat OCLN
- Species Reactivity
- Human, Mouse, Rat, Dog (Predicted), Pig (Predicted), Cow (Predicted), Sheep (Predicted)
- Applications
- ELISA, IHC-P, IHC-Fr, FC, ICC, IF
- Conjugate
- NONE
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01× TBS (pH 7.4) containing 50% Glycerol and 1% BSA.
- Preservative
- 0.03% Proclin 300
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- ELISA: 1/5000-1/10000
Immunohistochemistry-paraffin (IHC-P): 1/100-1/500
Immunohistochemistry-frozen (IHC-Fr): 1/100-1/500
Flow cytometry (FC): 1 μg per 1x 10⁶ cells
Immunocytochemistry (ICC): 1/100
Immunofluorescence (IF): 1/100-1/500
Target
- Alternative Names
- Occludin; Phosphatase 1, Regulatory Subunit 115; Tight Junction Protein Occludin TM4 Minus; Tight Junction Protein Occludin; PPP1R115; PTORCH1; BLCPMG;
- Gene ID
- 100506658
- UniProt ID
- Q16625
- Long Name
- Occludin
- Sequence Similarities
- Belongs to the ELL/occludin family.
- Cellular Localization
- Cell membrane; Cell junction; Tight junction
- Protein Refseq
- NP_001192183.1; NP_001192184.1; NP_002529.1
- Function
- May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
(Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "OCLN"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2734z | Mouse Anti-OCLN Recombinant Antibody (clone 34D5) | WB, ELISA, IF, IHC | Mouse IgG1, κ |
| MRO-1124-CN | Recombinant Rabbit Anti-OCLN Monoclonal Antibody (CBACN-410) | WB, IP, FC | Rabbit IgG |
| MRO-2091-CN | Rabbit Anti-OCLN Polyclonal Antibody (MRO-2091-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK1012 | Rabbit Anti-OCLN Recombinant Antibody (VS3-WK1012) | WB, IP, FC | Rabbit IgG |
| VS4-CJ64 | Recombinant Rabbit Anti-OCLN Antibody (clone 8H8) | ELISA, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2533 | Hi-Affi™ Recombinant Rabbit Anti-OCLN Monoclonal Antibody (DS2533AB) | WB, IP, FC | IgG |
| MOR-4657 | Hi-Affi™ Recombinant Rabbit Anti-OCLN Monoclonal Antibody (TH171DS) | WB, IF, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5068 | Anti-OCLN Immunohistochemistry Kit | IHC | |
| VS-0525-XY5069 | Anti-Mouse OCLN Immunohistochemistry Kit | IHC |
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