Rabbit Anti-OGA Recombinant Antibody (clone 8D12)
CAT#: ZG-0062U
The purified Rabbit Anti-OGA Recombinant Antibody (clone 8D12) displayed high specificity against the OGA protein of human.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-OGA Antibody (ZG-0062U) in WB
Positive WB detected in: Hela whole cell lysate, L02 whole cell lysate, HepG2 whole cell lysate. All lanes: MGEA5 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 103, 96, 77, 97 kDa
Observed band size: 130 kDa
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/76501/1703_G2_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in spermatogonia in seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36141/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36141/72132_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36141/72132_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36141/72132_A_9_5.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36141/72132_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198408-OGA
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Specifications
- Immunogen
- A synthesized peptide derived from human OGA
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human OGA
- Species Reactivity
- Human
- Clone
- 8D12
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
- Related Disease
- Danubian Endemic Familial Nephropathy and Gm2 Gangliosidosis
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot. Specific conditions should be optimized by the end user.
WB:1:500-1:5000
Target
- Alternative Names
- O-GlcNAcase; MEA5; MGEA5; NCOAT; protein O-GlcNAcase; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; beta-N-acetylglucosaminidase; beta-N-acetylhexosaminidase; beta-hexosaminidase; bifunctional protein NCOAT; hyaluronidase in meningioma; meningioma expressed antigen 5 (hyaluronidase); meningioma-expressed antigen 5; nuclear cytoplasmic O-GlcNAcase and acetyltransferase
- Gene ID
- 10724
- UniProt ID
- O60502
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "OGA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK500 | Rabbit Anti-OGA Recombinant Antibody (clone JG40-05) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5075 | Anti-OGA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5076 | Anti-Mouse OGA Immunohistochemistry Kit | IHC |
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