Rabbit Anti-P4HTM Recombinant Antibody (clone R06-1B0) (CAT#: VS3-FY2010)
We develop this astounding rabbit recombinant monoclonal antibody that specifically reacts with human P4HTM. This antibody is recommended for validation in immunohistochemistry (IHC) studies. It is suitable for use in a variety of assays, including WB, IHC-P, IP. This R06-1B0 antibody is validated for western blot analysis of HIF prolyl hydroxylases in Hela, A549, HL-60, U251, U87-MG lysates.
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WB
Figure 1 Anti-P4HTM Antibody (VS3-FY2010) in WB.
The HIF Prolyl Hydroxylases expression in Hela, A549, HL-60, U251, U87-MG lysates were detected using the anti-HIF Prolyl Hydroxylases by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human HIF prolyl hydroxylase
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human P4HTM
- Species Reactivity
- Human
- Clone
- R06-1B0
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 57 kDa, while the observed molecular weight stands at 43 kDa.
- Related Disease
- Hypotonia, Hypoventilation, Impaired Intellectual Development, Dysautonomia, Epilepsy, And Eye Abnormalities and Dysautonomia
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Prolyl 4-Hydroxylase, Transmembrane; Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum); Hypoxia-Inducible Factor Prolyl Hydroxylase 4; Prolyl Hydroxlase Domain-Containing 4; HIF-Prolyl Hydroxylase 4; HIF-PH4; P4H-TM; HPH-4; PH4; Hypoxia Inducible Factor Prolyl 4 Hydroxylase
- Gene ID
- 54681
- UniProt ID
- Q9NXG6
- Cellular Localization
- Endoplasmic reticulum
- Post Translation Modifications
- Glycosylated. (Q9NXG6-P4HTM_HUMAN)
Glycosylation at Asn348, Asn368, and Asn382 (NX_Q9NXG6 [NX_Q9NXG6-1])
Modification sites at PhosphoSitePlus (Q9NXG6)
Glycosylation from GlyGen (Q9NXG6) 6 sites, 2 O-linked glycans (3 sites)
- Protein Refseq
- XP_047304323.1; XP_047304324.1; XP_054202924.1; XP_054202925.1; NP_808807.2
- Function
- Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2569 | Hi-Affi™ Recombinant Rabbit Anti-P4HTM Monoclonal Antibody (DS2569AB) | IHC-P, WB | IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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