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PAIP1
Rabbit Anti-PAIP1 Recombinant Antibody (clone R08-8C6)

Rabbit Anti-PAIP1 Recombinant Antibody (clone R08-8C6) (CAT#: VS3-FY1814)

Name: Rabbit Anti-PAIP1 Recombinant Antibody (clone R08-8C6)
SKU: VS3-FY1814
Rating: 5 (1 reviews)

This R08-8C6 recombinant monoclonal antibody with high affinity for human PAIP1. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. To validate the QC result of the antibody, we recommend a dilution for the antibody by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). The specificity of the PAIP1 antibody was confirmed by western blot analysis of PAIP1 in Hela, 293T, mouse brain lysates.

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1 Anti-PAIP1 Antibody (VS3-FY1814) in WB.

Figure 1 Anti-PAIP1 Antibody (VS3-FY1814) in WB.

The PAIP1 expression in Hela, 293T, mouse brain lysates were detected using the anti-PAIP1 by western blot assay.

Subcellular Location

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/76187/1672_C9_33_selected.jpg

Normal Tissue

Figure 2 Cerebral cortex
(Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73653/162336_B_9_5.jpg

Figure 3 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73653/162336_A_7_3.jpg

Figure 4 Liver
(Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73653/162336_A_7_4.jpg

Figure 5 Kidney
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73653/162336_A_7_5.jpg

Figure 6 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73653/162336_A_4_6.jpg

RNA Expression

Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172239-PAIP1

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Synthetic peptide of human PAIP1

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse and Rat PAIP1

Species Reactivity
Human, Mouse, Rat

Clone
R08-8C6

Applications
Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation

MW
The calculated weight of the target protein is approximately 54 kDa, while the observed molecular weight stands at 54 kDa.

Product Property

Purification
Affinity Purified

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20

Target

Alternative Names
Poly(A) Binding Protein Interacting Protein 1; PABP-Interacting Protein 1; PAIP-1; Polyadenylate-Binding Protein-Interacting Protein 1; Poly(A)-Binding Protein-Interacting Protein 1; PABC1-Interacting Protein 1

Gene ID
10605

UniProt ID
Q9H074

Cellular Localization
Cytoplasm

Post Translation Modifications
Ubiquitination at Lys368 (NX_Q9H074 [NX_Q9H074-1])
Modification sites at PhosphoSitePlus (Q9H074)
Glycosylation from GlyGen (Q9H074) 2 sites, 1 O-linked glycan (2 sites)

Protein Refseq
NP_006442.2; NP_877590.1; NP_899152.1

Function
Acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs. Its stimulatory activity on translation is mediated via its action on PABPC1. Competes with PAIP2 for binding to PABPC1. Its association with EIF4A and PABPC1 may potentiate contacts between mRNA termini. May also be involved in translationally coupled mRNA turnover.

Isotype Control

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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MOB-1876MZ	Recombinant Mouse Anti-Human PAIP1 Antibody	ELISA, WB	Mouse antibody

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