Mouse Anti-PAK3 Recombinant Antibody (clone 4G8A5) (CAT#: VS7-0425-WR557)
This product is a mouse IgG1 antibody that binds to human PAK3. The antibody 4G8A5 recognizes specific amino acid domain of human PAK3 (AA: 1-100). It can be used for WB assay.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HAP1
(Immunofluorescent staining of human cell line HAP1 shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44791/1918_E6_28_cr5cb48efe99beb_selected.jpg
(Immunofluorescent staining of human cell line HAP1 shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44791/1918_E6_28_cr5cb48efe99beb_selected.jpg
Figure 2 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/ihc_selected.jpg
(Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/ihc_selected.jpg
Figure 3 Cerebral cortex
(Glial cells Staining: Not detected Neuronal cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/142917_B_7_5.jpg
(Glial cells Staining: Not detected Neuronal cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/142917_B_7_5.jpg
Figure 4 Cerebellum
(Purkinje cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/142917_B_9_8.jpg
(Purkinje cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/142917_B_9_8.jpg
Figure 5 Lymph node
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/142917_A_8_8.jpg
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/142917_A_8_8.jpg
Figure 6 RNA cell line category: Cell line enhanced (EFO-21, HAP1, HDLM-2, NTERA-2, SCLC-21H, SH-SY5Y, U-2197, U-251 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000077264-PAK3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000077264-PAK3
Specifications
- Immunogen
- Recombinant human PAK3 (AA: 1-100) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PAK3
- Species Reactivity
- Human
- Clone
- 4G8A5
- Molecule Class
- Serine/Threonine Kinase
- Applications
- WB
- Conjugate
- Unconjugated
- Related Disease
- Intellectual developmental disorder, X-linked 30 (XLID30)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- ARA; bPAK; MRX30; MRX47; OPHN3; PAK-3; XLID30; PAK3beta; beta-PAK
- Gene ID
- 5063
- UniProt ID
- O75914
- Long Name
- P21 (RAC1) Activated Kinase 3
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001121638.1
- Function
- Allosteric enzyme; Developmental protein; Kinase; Serine/threonine-protein kinase; Transferase
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Related Resources
Bladder Cancer
ErbB Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1537CT | Recombinant Mouse anti-Human PAK3 Monoclonal antibody (EML2324) | ICC/IF, WB | |
| VS3-WK72 | Mouse Anti-PAK3 Recombinant Antibody (clone F11-G8) | WB, ELISA | Mouse IgG1 |
| VS3-FY1092 | Recombinant Rabbit Anti-PAK3 (phospho Ser144) Antibody (clone R04-2A4) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
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