Mouse Anti-PAK3 Recombinant Antibody (clone 4G8A5) (CAT#: VS7-0425-WR557)
This product is a mouse IgG1 antibody that binds to human PAK3. The antibody 4G8A5 recognizes specific amino acid domain of human PAK3 (AA: 1-100). It can be used for WB assay.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HAP1
(Immunofluorescent staining of human cell line HAP1 shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44791/1918_E6_28_cr5cb48efe99beb_selected.jpg
(Immunofluorescent staining of human cell line HAP1 shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44791/1918_E6_28_cr5cb48efe99beb_selected.jpg
Figure 2 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/ihc_selected.jpg
(Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/ihc_selected.jpg
Figure 3 Cerebral cortex
(Glial cells Staining: Not detected Neuronal cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/142917_B_7_5.jpg
(Glial cells Staining: Not detected Neuronal cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/142917_B_7_5.jpg
Figure 4 Cerebellum
(Purkinje cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/142917_B_9_8.jpg
(Purkinje cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/142917_B_9_8.jpg
Figure 5 Lymph node
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/142917_A_8_8.jpg
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/142917_A_8_8.jpg
Figure 6 RNA cell line category: Cell line enhanced (EFO-21, HAP1, HDLM-2, NTERA-2, SCLC-21H, SH-SY5Y, U-2197, U-251 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000077264-PAK3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000077264-PAK3
Specifications
- Immunogen
- Recombinant human PAK3 (AA: 1-100) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PAK3
- Species Reactivity
- Human
- Clone
- 4G8A5
- Molecule Class
- Serine/Threonine Kinase
- Applications
- WB
- Conjugate
- Unconjugated
- Related Disease
- Intellectual developmental disorder, X-linked 30 (XLID30)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- ARA; bPAK; MRX30; MRX47; OPHN3; PAK-3; XLID30; PAK3beta; beta-PAK
- Gene ID
- 5063
- UniProt ID
- O75914
- Long Name
- P21 (RAC1) Activated Kinase 3
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001121638.1
- Function
- Allosteric enzyme; Developmental protein; Kinase; Serine/threonine-protein kinase; Transferase
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Related Resources
Bladder Cancer
ErbB Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1537CT | Recombinant Mouse anti-Human PAK3 Monoclonal antibody (EML2324) | ICC/IF, WB | |
| VS3-WK72 | Mouse Anti-PAK3 Recombinant Antibody (clone F11-G8) | WB, ELISA | Mouse IgG1 |
| VS3-FY1092 | Recombinant Rabbit Anti-PAK3 (phospho Ser144) Antibody (clone R04-2A4) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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