Rabbit Anti-PCYT1A Recombinant Antibody (clone 4F2)
CAT#: ZG-0112U
Rabbit anti-PCYT1A antibody (clone 4F2) displayed high specificity against the PCYT1A protein of Human.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-PCYT1A Antibody (ZG-0112U) in WB
Positive WB detected in: K562 whole cell lysate. All lanes: PCYT1A antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
IF
Figure 2 Rabbit Anti-PCYT1A Antibody (ZG-0112U) in IF
Immunofluorescence staining of Hela Cells with ZG-0112U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35428/382_E3_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35428/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35428/71903_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35428/71903_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35428/71903_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35428/71903_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35428/71903_A_4_6.jpg
Normal Tissue
Figure 8 Skin
Fibroblasts Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Keratinocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Langerhans Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Melanocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35428/71903_B_8_1.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (EFO-21)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000161217-PCYT1A
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Specifications
- Immunogen
- A synthesized peptide derived from human PCYT1A
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PCYT1A
- Species Reactivity
- Human
- Clone
- 4F2
- Applications
- ELISA, WB, IF
- Conjugate
- Unconjugated
- Related Disease
- Spondylometaphyseal Dysplasia With Cone-Rod Dystrophy and Leber Plus Disease
Product Property
- Purification
- Affinity Purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IF:1:20-1:200
Target
- Alternative Names
- CT; CTA; CCTA; CTPCT; PCYT1; SMDCRD; CCTalpha; choline-phosphate cytidylyltransferase A; CCT A; CCT-alpha; CT A; CTP:phosphocholine cytidylyltransferase A; CTP:phosphocholine cytidylyltransferase-alpha; choline-phosphate cytidylyltransferase alpha; phosphate cytidylyltransferase 1, choline, alpha; phosphorylcholine transferase A; phosphorylcholine transferase alpha
- Gene ID
- 5130
- UniProt ID
- P49585
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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