CytoStream™ Mouse Anti-PDCD1LG2 Recombinant Antibody (VS13-YC878)
CAT#: VS13-YC878
The CytoStream™ Mouse Anti-PDCD1LG2 Recombinant Antibody is specific for human, mouse and rat PDCD1LG2. PDCD1LG2 negatively regulates T cell activation and cytokine production, predicted to localize on the plasma membrane, identified as a tuberculosis biomarker. The anti-PDCD1LG2 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/104_F6_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity with granular pattern in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/200_H4_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_8_4.jpg
Normal Tissue
Figure 7 Pancreas
Exocrine glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Pancreatic endocrine cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_2_3.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13411/30825_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, HDLM-2, TIME
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197646-PDCD1LG2
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Specifications
- Immunogen
- Recombinant human CD273 expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human, Mouse and Rat PDCD1LG2
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, FC, ELISA
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunofluorescence (IF): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
ELISA: 1/10000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "Pdcd1lg2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-993-VHH | Recombinant Anti-Mouse Pdcd1lg2 VHH Single Domain Antibody | WB, ICC, ChiP, FA, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1012CL | Human Anti-PDCD1LG2 Recombinant Antibody (TAB-1012CL) | FC | Human IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1013CL | Mouse Anti-PDCD1LG2 Recombinant Antibody (TAB-1013CL) | ELISA | Mouse IgG |
| TAB-1014CL | Mouse Anti-PDCD1LG2 Recombinant Antibody (TAB-1014CL) | ELISA, FC | Mouse IgG |
| TAB-1013CL-S(P) | Mouse Anti-PDCD1LG2 Recombinant Antibody; scFv Fragment (TAB-1013CL-S(P)) | ELISA | Mouse scFv |
| TAB-1014CL-S(P) | Mouse Anti-PDCD1LG2 Recombinant Antibody; scFv Fragment (TAB-1014CL-S(P)) | ELISA, FC | Mouse scFv |
| TAB-1013CL-F(E) | Mouse Anti-PDCD1LG2 Recombinant Antibody; Fab Fragment (TAB-1013CL-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1012CL-S(P) | Human Anti-PDCD1LG2 Recombinant Antibody; scFv Fragment (TAB-1012CL-S(P)) | FC | Human scFv |
| TAB-1012CL-F(E) | Human Anti-PDCD1LG2 Recombinant Antibody; Fab Fragment (TAB-1012CL-F(E)) | FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0880MZ | Recombinant Mouse Anti-Human PDCD1LG2 Antibody (clone NJI25) | FC | Mouse antibody |
| ZG-0252U | Mouse Anti-PDCD1LG2 Recombinant Antibody (clone 7F11D11) | ELISA, WB, IHC, IF, FC, IP | Mouse IgG2b |
| VS3-WK1128 | Mouse Anti-PDCD1LG2 Recombinant Antibody (clone 3.2) | IF, FC | Mouse IgG1, κ |
| VS3-WK1129 | Mouse Anti-PDCD1LG2 Recombinant Antibody (VS3-WK1129) | IHC-P, WB | Mouse IgG1, κ |
| VS3-FY1109 | Recombinant Rabbit Anti-PDCD1LG2 Antibody (clone R02-2A1) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1839CQ | Recombinant Mouse Anti-PDCD1LG2 Antibody (MIH18) | FC, IHC, IF, BL | IgG1, κ |
| NEUT-1840CQ | Recombinant Rat Anti-Pdcd1lg2 Antibody (MAB0877) | BL, FC, IP, WB | IgG2 |
| NEUT-1841CQ | Recombinant Rat Anti-Pdcd1lg2 Antibody (TY25) | IHC-P, FC, WB, IP, BL, IHC-Fr | IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4357 | Hi-Affi™ Recombinant Rabbit Anti-PDCD1LG2 Monoclonal Antibody (SI447DS) | FC | IgG |
| MOR-4358 | Hi-Affi™ Recombinant Rabbit Anti-PDCD1LG2 Monoclonal Antibody (SI448DS) | FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0314-FY-S(P) | Human Anti-PDCD1LG2 Recombinant Antibody (clone CBLFY19); scFv Fragment | IHC, ELISA | Humanized scFv |
| HPAB-2927LY-S(P) | Mouse Anti-PDCD1LG2 Recombinant Antibody; scFv Fragment (HPAB-2927LY-S(P)) | ELISA | Mouse scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0314-FY-F(E) | Human Anti-PDCD1LG2 Recombinant Antibody (clone CBLFY19); Fab Fragment | IHC, ELISA | Humanized Fab |
| HPAB-2927LY-F(E) | Mouse Anti-PDCD1LG2 Recombinant Antibody; Fab Fragment (HPAB-2927LY-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY174 | CytoStream™ Mouse Anti-PDCD1LG2 Recombinant Antibody (clone 24F.10C12) | FC | Mouse IgG2a, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC279 | Recombinant Anti-PDCD1LG2 Vesicular Antibody, EV Displayed (VS-0425-YC279) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5255 | Anti-PDCD1LG2 Immunohistochemistry Kit | IHC |
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