Rabbit Anti-PDCD6IP Recombinant Antibody (clone 7E9) (CAT#: ZG-0504U)
Rabbit Anti-PDCD6IP Antibody (clone 7E9) displayed high specificity against the PDCD6IP protein of Human and Rat.
Specific Inquiry
WB
Figure 1 Rabbit Anti-PDCD6IP Antibody (ZG-0504U) in WB
Western Blot
Positive WB detected in: L02 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, U-87 whole cell lysate, Rat Liver whole cell lysate
All lanes: ALIX antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 97, 97, 31 kDa
Observed band size: 97 kDa
FC
Figure 2 Rabbit Anti-PDCD6IP Antibody (ZG-0504U) in FC
Overlay histogram showing Jurkat cells stained with ZG-0504U (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10, 000 events was performed.
Specifications
- Immunogen
- A synthesized peptide derived from human ALIX
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PDCD6IP
- Species Reactivity
- Human, Rat
- Clone
- 7E9
- Applications
- ELISA, WB, FC
- Conjugate
- Unconjugated
- Related Disease
- Neuronal Ceroid Lipofuscinosis.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, FC:1:20-1:200
Target
- Alternative Names
- Programmed cell death 6-interacting protein; PDCD6-interacting protein; ALG-2-interacting protein 1; ALG-2-interacting protein X; Hp95; PDCD6IP; AIP1 ALIX KIAA1375
- Gene ID
- 10015
- UniProt ID
- Q8WUM4
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2836z | Mouse Anti-PDCD6IP Recombinant Antibody (clone 27F7) | WB, ELISA, IHC | Mouse IgG1 |
| MOB-1299CT | Recombinant Mouse anti-Human PDCD6IP Monoclonal antibody (EML1259) | WB | |
| MRO-0057-CN | Recombinant Rabbit Anti-PDCD6IP Monoclonal Antibody (JM85-31) | WB, IHC, FC | Rabbit IgG |
| VS4-CJ68 | Recombinant Mouse Anti-PDCD6IP Antibody (clone 1E7E2) | ELISA, WB | Mouse IgG2b |
| VS3-FY1110 | Recombinant Rabbit Anti-PDCD6IP Antibody (clone R09-4B4) | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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