CytoStream™ Rabbit Anti-PDCD6IP Recombinant Antibody (VS13-YC882)
CAT#: VS13-YC882
The CytoStream™ Rabbit Anti-PDCD6IP Recombinant Antibody is specific for human and rat PDCD6IP. PDCD6IP encodes a protein functioning in cytokinesis and vesicle formation within the ESCRT pathway, with overexpression affecting apoptosis, interacting with PDCD6 in a calcium-dependent way, and featuring transcript variants. The anti-PDCD6IP antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11905/88_B12_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51604/781_E3_1_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11905/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16212/36104_A_5_2_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11905/29804_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11905/29804_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11905/29804_A_8_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11905/29804_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11905/29804_A_6_6.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000170248-PDCD6IP
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Specifications
- Immunogen
- Recombinant human ALIX, aa 1-160.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat PDCD6IP
- Species Reactivity
- Human, Rat
- Applications
- WB, IHC-P, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Programmed Cell Death 6 Interacting Protein; Programmed Cell Death 6-Interacting Protein; ALG-2-Interacting Protein X; PDCD6-Interacting Protein; AIP1; HP95; ALIX; Apoptosis-Linked Gene 2-Interacting Protein X;
- Gene ID
- 10015
- UniProt ID
- Q8WUM4
- Long Name
- Programmed Cell Death 6 Interacting Protein
- Cellular Localization
- Secreted; Cell junction; Cytoplasm; Cytoskeleton; Tight junction
- Protein Refseq
- NP_001155901.1; NP_001243121.1; NP_037506.2
- Function
- Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway requires the sequential function of ESCRT-O, -I, -II and -III complexes (PubMed:14739459).
The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis (PubMed:17853893, PubMed:17556548).
Adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis (PubMed:17853893, PubMed:17556548, PubMed:18641129).
May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP (PubMed:22660413).
By interacting with F-actin, PARD3 and TJP1 secures the proper assembly and positioning of actomyosin-tight junction complex at the apical sides of adjacent epithelial cells that defines a spatial membrane domain essential for the maintenance of epithelial cell polarity and barrier (By similarity).
(Microbial infection) Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function requires the interaction with CHMP4B. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as enveloped virus budding (HIV-1 and other lentiviruses).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2836z | Mouse Anti-PDCD6IP Recombinant Antibody (clone 27F7) | WB, ELISA, IHC | Mouse IgG1 |
| MOB-1299CT | Recombinant Mouse anti-Human PDCD6IP Monoclonal antibody (EML1259) | WB | |
| MRO-0057-CN | Recombinant Rabbit Anti-PDCD6IP Monoclonal Antibody (JM85-31) | WB, IHC, FC | Rabbit IgG |
| VS3-WK618 | Rabbit Anti-PDCD6IP Recombinant Antibody (clone JM85-31) | WB, ELISA | Rabbit IgG |
| VS4-CJ68 | Recombinant Mouse Anti-PDCD6IP Antibody (clone 1E7E2) | ELISA, WB | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC294 | AbPlus™ Anti-PDCD6IP Magnetic Beads (VS-0724-YC294) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5258 | Anti-PDCD6IP Immunohistochemistry Kit | IHC | |
| VS-0525-XY5259 | Anti-Mouse PDCD6IP Immunohistochemistry Kit | IHC |
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