SmartAb™ Recombinant Anti-PDGFB pH-dependent Antibody (VS-0825-YC280)
CAT#: VS-0825-YC280
The anti-PDGFB pH-dependent antibody (VS-0825-YC280) targets human PDGFB in a pH-dependent manner, with its affinity remaining high at neutral pH but weakening at a lower pH, such as within the acidic environment of endosomes. The anti-PDGFB acid-switched antibody was engineered by incorporating histidine residues into its variable regions. The introduction of histidine substitutions within its Complementarity-Determining Regions (CDRs) enables pH-responsive antigen binding, which leads to enhanced PDGFB clearance. The antibody operates in a cyclical manner, binding PDGFB in the extracellular fluid and releasing it in acidified endosomes. Human PDGFB (Platelet-Derived Growth Factor B) is a growth factor that promotes cell proliferation, migration, and survival. It is a key regulator of angiogenesis and is implicated in wound healing, fibrosis, and cancer.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11972/1592_D1_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human caudate
Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in granular pattern in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11972/ihc_selected.jpg
Normal Tissue
Figure 3 Placenta
Cytotrophoblasts Staining: Medium Intensity: Moderate Quantity:>75% Endothelial cells Staining: High Intensity: Strong Quantity:>75% Hofbauer cells Staining: Medium Intensity: Moderate Quantity:>75% Syncytiotrophoblasts - cell body Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11972/28202_A_1_7.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11972/28202_A_9_8.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11972/28202_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11972/28202_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18341/40769_A_5_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (A-431, EFO-21, HUVEC TERT2, SK-BR-3, TIME)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100311-PDGFB
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Specifications
- Immunogen
- Recombinant human PDGF-BB protein.
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human PDGFB
- Species Reactivity
- Human
- Applications
- ELISA, FC, IF, Block
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE and SEC-HPLC
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- For short-term storage, keep it at 4°C for up to two weeks. For long-term storage, divide the solution into aliquots and freeze them at -20°C or below.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "PDGFB"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-297 | Human Anti-PDGFB Recombinant Antibody scFv Fragment (PSBL-297) | WB, ELISA, FuncS | Human scFv |
| HPAB-0385-CN-S(P) | Human Anti-PDGFB Recombinant Antibody; scFv Fragment (HPAB-0385-CN-S(P)) | ELISA, FC, IF, Block | Human scFv |
| HPAB-0386-CN-S(P) | Rat Anti-PDGFB Recombinant Antibody; scFv Fragment (HPAB-0386-CN-S(P)) | ELISA, Block | Rat scFv |
| HPAB-0387-CN-S(P) | Rat Anti-PDGFB Recombinant Antibody; scFv Fragment (HPAB-0387-CN-S(P)) | ELISA, Block | Rat scFv |
| HPAB-0067-YJ-S(P) | Mouse Anti-PDGFB Recombinant Antibody (clone C1); scFv Fragment | ELISA, FuncS, Apop | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-297 | Human Anti-PDGFB Recombinant Antibody Fab Fragment (PFBL-297) | WB, ELISA, FuncS | Human Fab |
| HPAB-0385-CN-F(E) | Human Anti-PDGFB Recombinant Antibody; Fab Fragment (HPAB-0385-CN-F(E)) | ELISA, FC, IF, Block | Human Fab |
| HPAB-0386-CN-F(E) | Rat Anti-PDGFB Recombinant Antibody; Fab Fragment (HPAB-0386-CN-F(E)) | ELISA, Block | Rat Fab |
| HPAB-0387-CN-F(E) | Rat Anti-PDGFB Recombinant Antibody; Fab Fragment (HPAB-0387-CN-F(E)) | ELISA, Block | Rat Fab |
| HPAB-0388-CN-F(E) | Rat Anti-PDGFB Recombinant Antibody; Fab Fragment (HPAB-0388-CN-F(E)) | ELISA, Block | Rat Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1020CL | Anti-Human PDGFB Recombinant Antibody (F3) | ELISA | Rabbit mAb |
| TAB-1020CL-S(P) | Anti-Human PDGFB Recombinant Antibody scFv Fragment (F3) | ELISA | Rabbit mAb |
| TAB-1020CL-F(E) | Anti-Human PDGFB Recombinant Antibody Fab Fragment (F3) | ELISA | Rabbit mAb |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1021CL | Anti-Human PDGFB Recombinant Antibody (C12) | ELISA | Humanized antibody |
| TAB-1021CL-S(P) | Anti-Human PDGFB Recombinant Antibody scFv Fragment (C12) | ELISA | Humanized antibody |
| TAB-1021CL-F(E) | Anti-Human PDGFB Recombinant Antibody Fab Fragment (C12) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1049MZ | Recombinant Mouse Anti-Human PDGFB Antibody (clone NN0025-6G77) | IHC-P | Mouse antibody |
| HPAB-0385-CN | Human Anti-PDGFB Recombinant Antibody (HPAB-0385-CN) | ELISA, FC, IF, Block | Human IgG |
| HPAB-0386-CN | Rat Anti-PDGFB Recombinant Antibody (HPAB-0386-CN) | ELISA, Block | Rat IgG2b, κ |
| HPAB-0387-CN | Rat Anti-PDGFB Recombinant Antibody (HPAB-0387-CN) | ELISA, Block | Rat IgG2b, λ |
| HPAB-0067-YJ | Mouse Anti-PDGFB Recombinant Antibody (clone C1) | ELISA, FuncS, Apop | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2635 | Hi-Affi™ Recombinant Rabbit Anti-PDGFB Monoclonal Antibody (DS2635AB) | WB, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5273 | Anti-PDGFB Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC249 | Recombinant Anti-PDGFB Eliminating Antibody, pH-Sensitive (VS-0625-YC249) | Antigen-Sweeping In Vivo. | Human IgG |
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