Mouse Anti-PDGFRA Recombinant Antibody (clone 3B10-10E4-5B3)
CAT#: VS3-QX858
This product is a mouse antibody that recognizes PDGFRA. The antibody can be used in applications: IHC.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm, plasma membrane & cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4947/1030_B8_3_selected.jpg
Normal Tissue
Figure 2 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows moderate cytoplasmic positivity in megakaryocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18143/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18143/43112_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Enterocytes - Fibroblasts Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18143/43112_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18143/43112_A_7_5.jpg
Normal Tissue
Figure 6 Bone marrow
Hematopoietic cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18143/43112_B_5_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, BJ hTERT+, fHDF/TERT166, HHSteC, HSkMC)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134853-PDGFRA
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Specifications
- Immunogen
- Synthetic peptide conjugated to KLH.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Specificity
- PDGFRα
- Species Reactivity
- Human, Rat, Mouse
- Clone
- 3B10-10E4-5B3
- Applications
- IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- IHC: 1:100-1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-192 | Anti-Human PDGF-R alpha Recombinant Antibody (Olaratumab) | IF, IP, Neut, FuncS, ELISA, FC, ICC | IgG1 - kappa |
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| TAB-1063CL | Human Anti-PDGFRA Recombinant Antibody (TAB-1063CL) | FuncS | Human antibody |
| TAB-1063CL-S(P) | Human Anti-PDGFRA Recombinant Antibody; scFv Fragment (TAB-1063CL-S(P)) | ELISA | Human scFv |
| TAB-1063CL-F(E) | Human Anti-PDGFRA Recombinant Antibody; Fab Fragment (TAB-1063CL-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1848CQ | Recombinant Rat Anti-Pdgfra Antibody (APA5) | FC, FuncS, IHC-Fr, Neut, WB | IgG2a, κ |
| NEUT-1849CQ | Recombinant Mouse Anti-PDGFRA Antibody (CBL458) | WB, IHC, Neut | IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2637 | Hi-Affi™ Recombinant Rabbit Anti-PDGFRA Monoclonal Antibody (DS2637AB) | WB | IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0232LY-S(P) | Human Anti-PDGFRA Recombinant Antibody (clone 2.175.3); scfv Fragment | Inhib, FC | Human scfv |
| HPAB-0233LY-S(P) | Human Anti-PDGFRA Recombinant Antibody (clone 2.451.1.1); scfv Fragment | Inhib, FC | Human scfv |
| HPAB-0234LY-S(P) | Human Anti-PDGFRA Recombinant Antibody (clone 2.449.1.3); scfv Fragment | Inhib, FC | Human scfv |
| HPAB-0235LY-S(P) | Human Anti-PDGFRA Recombinant Antibody (clone 2.998.2); scfv Fragment | Inhib, FC | Human scfv |
| HPAB-0236LY-S(P) | Human Anti-PDGFRA Recombinant Antibody (clone 2.84.3); scfv Fragment | Inhib, FC | Human scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0244LY-F(E) | Human Anti-PDGFRA Recombinant Antibody (clone 2.1954.2); Fab Fragment | Inhib, FC | Human Fab |
| HPAB-0245LY-F(E) | Human Anti-PDGFRA Recombinant Antibody (clone 3.23.2); Fab Fragment | Inhib, FC | Human Fab |
| HPAB-0246LY-F(E) | Human Anti-PDGFRA Recombinant Antibody (clone 3.87.1.1); Fab Fragment | Inhib, FC | Human Fab |
| HPAB-0247LY-F(E) | Human Anti-PDGFRA Recombinant Antibody (clone 3.341.1); Fab Fragment | Inhib, FC | Human Fab |
| HPAB-0248LY-F(E) | Human Anti-PDGFRA Recombinant Antibody (clone 3.472.1.1); Fab Fragment | Inhib, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-192 | Afuco™ Anti-PDGFRA ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-192) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-878 | Afuco™ Anti-PDGFRA ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-878) | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY164 | Human Anti-PDGFRA (clone 2.175.3) scFv-Fc Chimera | Inhib, FC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC216 | Recombinant Anti-PDGFRA Vesicular Antibody, EV Displayed (VS-0425-YC216) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5274 | Anti-PDGFRA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5275 | Anti-Mouse PDGFRA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5276 | Anti-Rat PDGFRA Immunohistochemistry Kit | IHC |
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