Mouse Anti-PDPK1 Recombinant Antibody (clone 3H3D9) (CAT#: VS7-0425-WR592)
This product is a mouse IgG1 antibody that binds to human PDPK1. The antibody 3H3D9 recognizes specific amino acid domain of human PDPK1 (AA: 457-556). It can be used for WB, ICC, and FC assays.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35199/if_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35199/if_selected.jpg
Figure 2 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4272/ihc_selected.jpg
(Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4272/ihc_selected.jpg
Figure 3 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35199/138206_A_7_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35199/138206_A_7_3.jpg
Figure 4 Fallopian tube
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35199/138206_A_7_7.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35199/138206_A_7_7.jpg
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000140992-PDPK1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000140992-PDPK1
Specifications
- Immunogen
- Recombinant human PDPK1 (AA: 457-556) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PDPK1
- Species Reactivity
- Human
- Clone
- 3H3D9
- Molecule Class
- Serine/Threonine Kinase
- Applications
- WB, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PDPK1; PDK1; PkB Kinase Like Gene 1; PDPK2P; HPDK1; EC 2.7.11.1; 3-Phosphoinositide Dependent Protein Kinase-1; 3-Phosphoinositide-Dependent Protein Kinase 2 Pseudogene; EC 2.7.11; PRO0461
- Gene ID
- 5170
- UniProt ID
- O15530
- Long Name
- 3-Phosphoinositide Dependent Protein Kinase 1
- Cellular Localization
- Cell junction; Cell membrane; Cytoplasm; Membrane; Nucleus
- Post Translation Modifications
- Acetylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001248745.1
- Function
- Activator; Kinase; Serine/threonine-protein kinase; Transferase
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Related Resources
Insulin Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "PDPK1"
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IgG Antibody Products
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Non-Human Primate (NHP) Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2837z | Mouse Anti-PDPK1 Recombinant Antibody (clone 29H1) | WB, ELISA, FC, IF, IHC | Mouse IgG1 |
| MRO-1201-CN | Recombinant Rabbit Anti-PDPK1 Monoclonal Antibody (CBACN-440) | WB, IF, IHC | Rabbit IgG |
| VS3-CJ142 | Rabbit Anti-PDPK1 Recombinant Antibody (VS3-CJ142) | WB, ICC, IF, IHC | Rabbit IgG |
| VS3-FY1121 | Recombinant Rabbit Anti-PDPK1 Antibody (clone R03-7H6) | WB | Rabbit IgG |
| VS7-0425-WR591 | Mouse Anti-PDPK1 Recombinant Antibody (clone 7E4G11) | WB, IHC, ICC, FC | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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