Mouse Anti-PDPK1 Recombinant Antibody (clone 3H3D9)
CAT#: VS7-0425-WR592
This product is a mouse IgG1 antibody that binds to human PDPK1. The antibody 3H3D9 recognizes specific amino acid domain of human PDPK1 (AA: 457-556). It can be used for WB, ICC, and FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35199/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4272/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35199/138206_A_7_3.jpg
Normal Tissue
Figure 4 Fallopian tube
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35199/138206_A_7_7.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000140992-PDPK1
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Specifications
- Immunogen
- Recombinant human PDPK1 (AA: 457-556) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PDPK1
- Species Reactivity
- Human
- Clone
- 3H3D9
- Molecule Class
- Serine/Threonine Kinase
- Applications
- WB, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PDPK1; PDK1; PkB Kinase Like Gene 1; PDPK2P; HPDK1; EC 2.7.11.1; 3-Phosphoinositide Dependent Protein Kinase-1; 3-Phosphoinositide-Dependent Protein Kinase 2 Pseudogene; EC 2.7.11; PRO0461
- Gene ID
- 5170
- UniProt ID
- O15530
- Long Name
- 3-Phosphoinositide Dependent Protein Kinase 1
- Cellular Localization
- Cell junction; Cell membrane; Cytoplasm; Membrane; Nucleus
- Post Translation Modifications
- Acetylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001248745.1
- Function
- Activator; Kinase; Serine/threonine-protein kinase; Transferase
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Related Signaling Pathways
Insulin Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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