Mouse Anti-PDPK1 Recombinant Antibody (clone 7E4G11)
CAT#: VS7-0425-WR591
This product is a mouse IgG1 antibody that binds to human PDPK1. The antibody 7E4G11 recognizes specific amino acid domain of human PDPK1 (AA: 457-556). It can be used for WB, IHC, ICC, and FC assays. It cross reacts with mouse, monkey, and rat.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35199/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4272/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35199/138206_A_7_3.jpg
Normal Tissue
Figure 4 Fallopian tube
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35199/138206_A_7_7.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000140992-PDPK1
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Specifications
- Immunogen
- Recombinant human PDPK1 (AA: 457-556) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PDPK1
- Species Reactivity
- Human, Mouse, Monkey, Rat
- Clone
- 7E4G11
- Molecule Class
- Serine/Threonine Kinase
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PDPK1; PDK1; PkB Kinase Like Gene 1; PDPK2P; HPDK1; EC 2.7.11.1; 3-Phosphoinositide Dependent Protein Kinase-1; 3-Phosphoinositide-Dependent Protein Kinase 2 Pseudogene; EC 2.7.11; PRO0461
- Gene ID
- 5170
- UniProt ID
- O15530
- Long Name
- 3-Phosphoinositide Dependent Protein Kinase 1
- Cellular Localization
- Cell junction; Cell membrane; Cytoplasm; Membrane; Nucleus
- Post Translation Modifications
- Acetylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001248745.1
- Function
- Activator; Kinase; Serine/threonine-protein kinase; Transferase
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Related Signaling Pathways
Insulin Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "Clone 7E4G11"
- CAT
- Product Name
See other products for "PDPK1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2837z | Mouse Anti-PDPK1 Recombinant Antibody (clone 29H1) | WB, ELISA, FC, IF, IHC | Mouse IgG1 |
| VS3-XY1234 | Mouse Anti-PDPK1 Recombinant Antibody (clone 3B11H2) | ELISA, WB, FC, ICC | Mouse IgG1 |
| VS3-XY1235 | Mouse Anti-PDPK1 Recombinant Antibody (clone 3H6) | ELISA, WB, FC, ICC, IHC | Mouse IgG1 |
| VS3-FY1121 | Recombinant Rabbit Anti-PDPK1 Antibody (clone R03-7H6) | WB | Rabbit IgG |
| VS7-0425-WR592 | Mouse Anti-PDPK1 Recombinant Antibody (clone 3H3D9) | WB, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2649 | Hi-Affi™ Recombinant Rabbit Anti-PDPK1 Monoclonal Antibody (DS2649AB) | WB, IP, IHC-P, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY376 | Mouse Anti-NHP PDPK1 Recombinant Antibody (clone 7E4G11) | WB, IHC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1612 | Anti-PDPK1 Immunohistochemistry Kit | IHC |
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