Mouse Anti-PDPN Recombinant Antibody (clone PMab-1) (CAT#: FAMAB-1870CQ)

Figure 1 Immunostaining for podoplanin and FLAG on cultured cells.

The CHO and B16-F10 cells were exposed to a cocktail of PMab-1(green) and anti-FLAG (red) (PMab-1-FLAG). The HSC3 and LEC cells were exposed to a cocktail of NZ-1.2 (green) and anti-FLAG (NZ-1.2-FLAG). The nuclei were counter stained with DAPI. The CHO was not stained by PMab-1 or anti-FLAG. The B16-F10 was stained by PMab-1 at the cytoplasm but not by anti-FLAG. The HSC3 was not stained by NZ-1.2 or anti-FLAG but LEC was stained by NZ-1.2 at the cell-celljunction and the cytoplasm

Kaji, C., Tsujimoto, Y., Kaneko, M. K., Kato, Y., & Sawa, Y. (2012). Immunohistochemical examination of novel rat monoclonal antibodies against mouse and human podoplanin. Acta Histochemica et Cytochemica, 12008.

Figure 2 Immunostaining for podoplanin on mouse kidney and lung tissue.

In the glomerulus of the renal cortex, the merged image indicates that the PMab-1-reacting region on the glomerular capillary (GA) (green) coincides with the Mab-8.1.1-reacting region (red). The PMab-1 andMab-8.1.1 did not react to uriniferous tubules (UT). In the lung, the merged image indicates that the pulmonary pleura (PP) and a part of the pulmonary alveoli (PA) were immunostained by PMab-1 (green). The immunoreaction of Mab-8.1.1 (red) to alveolar cells was stronger thanPMab-1 and the Mab-8.1.1 specificity was broader comparing with PMab-1. Nuclei were counterstained using DAPI. Bar=20 μm.

Kaji, C., Tsujimoto, Y., Kaneko, M. K., Kato, Y., & Sawa, Y. (2012). Immunohistochemical examination of novel rat monoclonal antibodies against mouse and human podoplanin. Acta Histochemica et Cytochemica, 12008.