Mouse Anti-PDPN Recombinant Antibody (clone PMab-1) (CAT#: FAMAB-1870CQ)
This product is a recombinant mouse antibody that recognizes PDPN. The antibody was purified by affinity chromatography. It can be used in WB, Block, ELISA, IHC.
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Figure 1 Immunostaining for podoplanin and FLAG on cultured cells.
The CHO and B16-F10 cells were exposed to a cocktail of PMab-1(green) and anti-FLAG (red) (PMab-1-FLAG). The HSC3 and LEC cells were exposed to a cocktail of NZ-1.2 (green) and anti-FLAG (NZ-1.2-FLAG). The nuclei were counter stained with DAPI. The CHO was not stained by PMab-1 or anti-FLAG. The B16-F10 was stained by PMab-1 at the cytoplasm but not by anti-FLAG. The HSC3 was not stained by NZ-1.2 or anti-FLAG but LEC was stained by NZ-1.2 at the cell-celljunction and the cytoplasm
Kaji, C., Tsujimoto, Y., Kaneko, M. K., Kato, Y., & Sawa, Y. (2012). Immunohistochemical examination of novel rat monoclonal antibodies against mouse and human podoplanin. Acta Histochemica et Cytochemica, 12008.
IHC
Figure 2 Immunostaining for podoplanin on mouse kidney and lung tissue.
In the glomerulus of the renal cortex, the merged image indicates that the PMab-1-reacting region on the glomerular capillary (GA) (green) coincides with the Mab-8.1.1-reacting region (red). The PMab-1 andMab-8.1.1 did not react to uriniferous tubules (UT). In the lung, the merged image indicates that the pulmonary pleura (PP) and a part of the pulmonary alveoli (PA) were immunostained by PMab-1 (green). The immunoreaction of Mab-8.1.1 (red) to alveolar cells was stronger thanPMab-1 and the Mab-8.1.1 specificity was broader comparing with PMab-1. Nuclei were counterstained using DAPI. Bar=20 μm.
Kaji, C., Tsujimoto, Y., Kaneko, M. K., Kato, Y., & Sawa, Y. (2012). Immunohistochemical examination of novel rat monoclonal antibodies against mouse and human podoplanin. Acta Histochemica et Cytochemica, 12008.
Specifications
- Immunogen
- Synthetic peptide mpp38-51 (GDGMVPPGIEDKIT) of the PLAG domain of mouse podoplanin
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Mouse podoplanin
- Species Reactivity
- Mouse
- Clone
- PMab-1
- Applications
- WB, Block, ELISA, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Podoplanin; Lung Type I Cell Membrane Associated Glycoprotein; Glycoprotein 36; PA2.26 Antigen; T1-Alpha; AGGRUS; GP36; T1A; Lung Type-I Cell Membrane-Associated Glycoprotein (T1A-2); Glycoprotein, 36-KD; HT1alpha-1;
- Gene ID
- 14726
- UniProt ID
- Q62011
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1598z | Mouse Anti-PDPN Recombinant Antibody (clone 26C11) | WB, FC, ICC, IF, IHC | Mouse IgG1, κ |
| PABL-655 | Mouse Anti-PDPN Recombinant Antibody (PABL-655) | WB, FuncS | Mouse IgG2a |
| HPAB-0502-CN | Mouse Anti-PDPN Recombinant Antibody (HPAB-0502-CN) | WB, ELISA, FC, IP | Mouse IgG1, κ |
| HPAB-0503-CN | Human Anti-PDPN Recombinant Antibody (HPAB-0503-CN) | WB, ELISA, FC, IP | Chimeric (mouse/human) IgG1 |
| HPAB-0504-CN | Mouse Anti-PDPN Recombinant Antibody (HPAB-0504-CN) | WB, ELISA, FC, IP | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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