Rabbit Anti-PDPN Recombinant Antibody (clone R77-1S-2)
CAT#: VS3-FY2118
Clone R77-1S-2 is a rabbit recombinant antibody that exhibits specific binding to human PDPN protein, a calculated weight of 17 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R77-1S-2 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line EFO-21
Immunofluorescent staining of human cell line EFO-21 shows localization to plasma membrane & cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7949/1907_B7_4_selected.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8376/24701_A_9_3.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: Low Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8376/24701_A_4_6.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (AF22, EFO-21, HAP1, HHSteC, NTERA-2, SuSa, TIME)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162493-PDPN
❮
❯
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PDPN
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R77-1S-2
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 17 kDa, while the observed molecular weight stands at 36kDa.
- Related Disease
- Lymphangioma and Angiosarcoma
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Podoplanin; Lung Type I Cell Membrane Associated Glycoprotein; Glycoprotein 36; PA2.26 Antigen; T1-Alpha; AGGRUS; GP36; T1A; Lung Type-I Cell Membrane-Associated Glycoprotein (T1A-2); Glycoprotein, 36-KD; HT1alpha-1
- Gene ID
- 10630
- UniProt ID
- Q86YL7
- Sequence Similarities
- Belongs to the podoplanin family.
- Cellular Localization
- Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane
- Post Translation Modifications
- Extensively O-glycosylated.
Contains sialic acid residues.
O-glycosylation is necessary for platelet aggregation activity.
Disialylated at Thr-52; sialic acid is critical for platelet-aggregating activity and for CLEC1B interaction (PubMed:17222411, 25458834).
(Q86YL7-PDPN_HUMAN)
The N-terminus is blocked. (Q86YL7-PDPN_HUMAN)
Cleaved by a metalloprotease within its extracellular (EC) domain, generating a membrane-bound C-terminal fragment (PCTF33) and an extracellular fragment.
The resulting membrane-bound C-terminal fragment (PCTF33) is further processed between Val-150 and Val-151 by PSEN1/gamma-secretase generating the intracellular domain of podoplanin (PICD).
- Protein Refseq
- XP_006710358.1; XP_024307172.1; XP_047290427.1; XP_054189868.1; XP_054189869.1
- Function
- Through MSN or EZR interaction promotes epithelial-mesenchymal transition (EMT) leading to ERZ phosphorylation and triggering RHOA activation leading to cell migration increase and invasiveness.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY2118. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PDPN"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-650 | Mouse Anti-PDPN Recombinant Antibody; Fab Fragment (PFBL-650) | WB, FuncS | Mouse Fab |
| HPAB-0502-CN-F(E) | Mouse Anti-PDPN Recombinant Antibody; Fab Fragment (HPAB-0502-CN-F(E)) | WB, ELISA, FC, IP | Mouse Fab |
| HPAB-0503-CN-F(E) | Human Anti-PDPN Recombinant Antibody; Fab Fragment (HPAB-0503-CN-F(E)) | WB, ELISA, FC, IP | Human Fab |
| HPAB-0504-CN-F(E) | Mouse Anti-PDPN Recombinant Antibody; Fab Fragment (HPAB-0504-CN-F(E)) | WB, ELISA, FC, IP | Mouse Fab |
| HPAB-0505-CN-F(E) | Human Anti-PDPN Recombinant Antibody; Fab Fragment (HPAB-0505-CN-F(E)) | WB, ELISA, FC, IP | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1055CL-S(P) | Rat Anti-PDPN Recombinant Antibody; scFv Fragment (TAB-1055CL-S(P)) | SDS-PAGE, WB, FC | Rat scFv |
| TAB-0567CL | Mouse Anti-PDPN Recombinant Antibody (TAB-0567CL) | WB, FC | Mouse IgG |
| TAB-0568CL | Mouse Anti-PDPN Recombinant Antibody (TAB-0568CL) | WB, SPR, ELISA, FC, Inhib, FuncS | Mouse IgG |
| TAB-0567CL-F(E) | Mouse Anti-PDPN Recombinant Antibody; Fab Fragment (TAB-0567CL-F(E)) | WB, FC | Mouse Fab |
| TAB-0568CL-F(E) | Mouse Anti-PDPN Recombinant Antibody; Fab Fragment (TAB-0568CL-F(E)) | WB, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1851CQ | Recombinant Hamster Anti-Pdpn Antibody (8.1.1) | BL, IF, WB, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2650 | Hi-Affi™ Recombinant Rabbit Anti-PDPN Monoclonal Antibody (DS2650AB) | FC, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0502-CN | Mouse Anti-PDPN Recombinant Antibody (HPAB-0502-CN) | WB, ELISA, FC, IP | Mouse IgG1, κ |
| HPAB-0503-CN | Human Anti-PDPN Recombinant Antibody (HPAB-0503-CN) | WB, ELISA, FC, IP | Chimeric (mouse/human) IgG1 |
| HPAB-0504-CN | Mouse Anti-PDPN Recombinant Antibody (HPAB-0504-CN) | WB, ELISA, FC, IP | Mouse IgG |
| HPAB-0505-CN | Human Anti-PDPN Recombinant Antibody (HPAB-0505-CN) | WB, ELISA, FC, IP | Chimeric (mouse/human) IgG1 |
| HPAB-0417-YC | Rat Anti-PDPN Recombinant Antibody (HPAB-0417-YC) | WB, FC, IHC, IP, Cyt | Rat IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0502-CN-S(P) | Mouse Anti-PDPN Recombinant Antibody; scFv Fragment (HPAB-0502-CN-S(P)) | WB, ELISA, FC, IP | Mouse scFv |
| HPAB-0503-CN-S(P) | Mouse Anti-PDPN Recombinant Antibody; scFv Fragment (HPAB-0503-CN-S(P)) | WB, ELISA, FC, IP | Mouse scFv |
| HPAB-0504-CN-S(P) | Mouse Anti-PDPN Recombinant Antibody; scFv Fragment (HPAB-0504-CN-S(P)) | WB, ELISA, FC, IP | Mouse scFv |
| HPAB-0505-CN-S(P) | Mouse Anti-PDPN Recombinant Antibody; scFv Fragment (HPAB-0505-CN-S(P)) | WB, ELISA, FC, IP | Mouse scFv |
| HPAB-0417-YC-S(P) | Rat Anti-PDPN Recombinant Antibody; scFv Fragment (HPAB-0417-YC-S(P)) | WB, FC, IHC, IP, Cyt | Rat scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| CBP-215YJL | Recombinant Human Podoplanin Protein | Positive Control, Immunogen, SDS-PAGE, WB, ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1293 | AbPlus™ Anti-PDPN Magnetic Beads (VS-0724-YC1293) | IP, Protein Purification | |
| VS-0424-XY210 | AbPlus™ Anti-PDPN Magnetic Beads (VS-0424-XY210) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC272 | Recombinant Anti-PDPN Vesicular Antibody, EV Displayed (VS-0425-YC272) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5296 | Anti-Mouse PDPN Immunohistochemistry Kit | IHC | |
| VS-0525-XY5297 | Anti-Rat PDPN Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application:
-2.jpg)
Application: IB, ELISA, FC, FuncS
Application: FC, ADCC, CDC, Inhib
Application: ELISA, Activ, Block
Application: ELISA, IHC, IF, IP
Application: EM, ELISA, ICC, IHC-Fr, IHC-P, WB
Application: WB, IHC-Fr, IHC-P, ICC, IF
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
