Rabbit Anti-PDPN Recombinant Antibody (clone R77-1S-2) (CAT#: VS3-FY2118)
Clone R77-1S-2 is a rabbit recombinant antibody that exhibits specific binding to human PDPN protein, a calculated weight of 17 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R77-1S-2 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line EFO-21
(Immunofluorescent staining of human cell line EFO-21 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7949/1907_B7_4_selected.jpg
(Immunofluorescent staining of human cell line EFO-21 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7949/1907_B7_4_selected.jpg
Figure 2 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8376/24701_A_9_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8376/24701_A_9_3.jpg
Figure 3 Testis
(Leydig cells Staining: Low Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8376/24701_A_4_6.jpg
(Leydig cells Staining: Low Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8376/24701_A_4_6.jpg
Figure 4 RNA cell line category: Cell line enhanced (AF22, EFO-21, HAP1, HHSteC, NTERA-2, SuSa, TIME)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162493-PDPN
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162493-PDPN
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PDPN
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R77-1S-2
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 17 kDa, while the observed molecular weight stands at 36kDa.
- Related Disease
- Lymphangioma and Angiosarcoma
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Podoplanin; Lung Type I Cell Membrane Associated Glycoprotein; Glycoprotein 36; PA2.26 Antigen; T1-Alpha; AGGRUS; GP36; T1A; Lung Type-I Cell Membrane-Associated Glycoprotein (T1A-2); Glycoprotein, 36-KD; HT1alpha-1
- Gene ID
- 10630
- UniProt ID
- Q86YL7
- Sequence Similarities
- Belongs to the podoplanin family.
- Cellular Localization
- Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane
- Post Translation Modifications
- Extensively O-glycosylated.
Contains sialic acid residues.
O-glycosylation is necessary for platelet aggregation activity.
Disialylated at Thr-52; sialic acid is critical for platelet-aggregating activity and for CLEC1B interaction (PubMed:17222411, 25458834).
(Q86YL7-PDPN_HUMAN)
The N-terminus is blocked. (Q86YL7-PDPN_HUMAN)
Cleaved by a metalloprotease within its extracellular (EC) domain, generating a membrane-bound C-terminal fragment (PCTF33) and an extracellular fragment.
The resulting membrane-bound C-terminal fragment (PCTF33) is further processed between Val-150 and Val-151 by PSEN1/gamma-secretase generating the intracellular domain of podoplanin (PICD).
- Protein Refseq
- XP_006710358.1; XP_024307172.1; XP_047290427.1; XP_054189868.1; XP_054189869.1
- Function
- Through MSN or EZR interaction promotes epithelial-mesenchymal transition (EMT) leading to ERZ phosphorylation and triggering RHOA activation leading to cell migration increase and invasiveness.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-655 | Mouse Anti-PDPN Recombinant Antibody (PABL-655) | WB, FuncS | Mouse IgG2a |
| MOB-1618CT | Recombinant Mouse anti-Human PDPN Monoclonal antibody (29I6) | FC, ICC/IF, IHC-Fr, IHC-P, WB | |
| MOB-066CQ | Golden Syrian Hamster Anti-Podoplanin Antibody | WB, FC, ICC/IF, IHC-Fr, IHC-P | Golden Syrian Hamster IgG |
| MRO-1252-CN | Recombinant Rabbit Anti-PDPN Monoclonal Antibody (JM31-21) | WB, IHC | Rabbit IgG |
| FAMAB-1296CQ | Mouse Anti-PDPN Recombinant Antibody (clone PMab-2) | WB, FC, IHC | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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