Rabbit Anti-Phospho-MAPK1/MAPK3 Recombinant Antibody (clone G15-B)
CAT#: ZG-0408U
Rabbit anti-Phospho-MAPK1/MAPK3 antibody (clone G15-B) displayed high specificity against the Phospho-MAPK1/MAPK3 protein of Human, mouse and rat.
Tested Data
WB
Figure 1 Rabbit Anti-Phospho-MAPK1/MAPK3 Antibody (ZG-0408U) in WB
Western blot analysis of Erk 1,2 activation in untreated PC12 cells (A), cells treated with EGF- 100ng/ml, 5min (B), PMA-100nM, 30min (C) in serum free DMEM, and EGF-100ng/ml, 5 min with 10% FBS in DMEM (D). Wells were equally loaded with 50μg of whole cell lysate proteins/well.
IF
Figure 2 Rabbit Anti-Phospho-MAPK1/MAPK3 Antibody (ZG-0408U) in IF
Representative pictures of phospho-Erk1,2 expression in HEK293 cells, (hydrogen peroxide treated cells) visualized with rabbit anti-phospho-Erk1,2 antibody. Primary antibody dilution 1:100.
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Specifications
- Immunogen
- Peptide derived from the protein area including conserved pT-E-pY motif of activated Erk 1,2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Phospho-MAPK1/MAPK3
- Species Reactivity
- Human, Mouse, Rat
- Clone
- G15-B
- Applications
- ELISA, WB, IP, ICC
- Conjugate
- Unconjugated
- Related Disease
- Noonan Syndrome 13, Specific Learning Disability, Bile Duct Cancer and Pancreatic Adenocarcinoma.
Product Property
- Purification
- Affinity Purified
- Buffer
- 20 mM Tris-HCl, pH 8.0, 10 mg/ml BSA and 0.05% sodium azide
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunoprecipitation, Immunocytochemistry.
Target
- Alternative Names
- ERK; p38; p40; p41; ERK2; ERT1; NS13; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK; mitogen-activated protein kinase 1; MAP kinase 1; MAP kinase 2; MAPK 2; extracellular signal-regulated kinase 2; mitogen-activated protein kinase 2; protein tyrosine kinase ERK2; EC 2.7.11.24; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK; mitogen-activated protein kinase 3; MAPK 1; extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; insulin-stimulated MAP2 kinase; microtubule-associated protein 2 kinase; EC 2.7.11.24
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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