Rabbit Anti-Phospho-MAPK1/MAPK3 Recombinant Antibody (clone G15-B) (CAT#: ZG-0408U)
Rabbit anti-Phospho-MAPK1/MAPK3 antibody (clone G15-B) displayed high specificity against the Phospho-MAPK1/MAPK3 protein of Human, mouse and rat.
Specific Inquiry
WB
Figure 1 Rabbit Anti-Phospho-MAPK1/MAPK3 Antibody (ZG-0408U) in WB
Western blot analysis of Erk 1,2 activation in untreated PC12 cells (A), cells treated with EGF- 100ng/ml, 5min (B), PMA-100nM, 30min (C) in serum free DMEM, and EGF-100ng/ml, 5 min with 10% FBS in DMEM (D). Wells were equally loaded with 50μg of whole cell lysate proteins/well.
IF
Figure 2 Rabbit Anti-Phospho-MAPK1/MAPK3 Antibody (ZG-0408U) in IF
Representative pictures of phospho-Erk1,2 expression in HEK293 cells, (hydrogen peroxide treated cells) visualized with rabbit anti-phospho-Erk1,2 antibody. Primary antibody dilution 1:100.
Specifications
- Immunogen
- Peptide derived from the protein area including conserved pT-E-pY motif of activated Erk 1,2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Phospho-MAPK1/MAPK3
- Species Reactivity
- Human, Mouse, Rat
- Clone
- G15-B
- Applications
- ELISA, WB, IP, ICC
- Conjugate
- Unconjugated
- Related Disease
- Noonan Syndrome 13, Specific Learning Disability, Bile Duct Cancer and Pancreatic Adenocarcinoma.
Product Property
- Purification
- Affinity Purified
- Buffer
- 20 mM Tris-HCl, pH 8.0, 10 mg/ml BSA and 0.05% sodium azide
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunoprecipitation, Immunocytochemistry.
Target
- Alternative Names
- ERK; p38; p40; p41; ERK2; ERT1; NS13; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK; mitogen-activated protein kinase 1; MAP kinase 1; MAP kinase 2; MAPK 2; extracellular signal-regulated kinase 2; mitogen-activated protein kinase 2; protein tyrosine kinase ERK2; EC 2.7.11.24; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK; mitogen-activated protein kinase 3; MAPK 1; extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; insulin-stimulated MAP2 kinase; microtubule-associated protein 2 kinase; EC 2.7.11.24
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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