Rabbit Anti-Phospho-RAF1 (S621) Recombinant Antibody (clone 1C2)
CAT#: ZG-0539U
Rabbit Anti-Phospho-RAF1 (S621) Antibody (clone 1C2) displayed high specificity against the RAF1 protein of Human.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-Phospho-RAF1 (S621) Antibody (ZG-0539U) in WB
Western Blot
Positive WB detected in Hela whole cell lysate(treated with Calyculin A or EGF)
All lanes Phospho-RAF1 antibody at 1.525μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 73 KDa
Observed band size: 73 KDa
IF
Figure 2 Rabbit Anti-Phospho-RAF1 (S621) Antibody (ZG-0539U) in IF
Immunofluorescence staining of HepG2 cells(treated with 50mM Calyculin A for 30min) with ZG-0539U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2639/1867_B3_30_cr5b68189b791a9_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_5_3_selected.jpg
Normal Tissue
Figure 3 IHC staining of human gall bladder
Immunohistochemical staining of human gall bladder shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19291/43320_A_5_4_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_8_8.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2640/8654_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132155-RAF1
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Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-RAF1 (S621)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Phospho-RAF1 (S621)
- Species Reactivity
- Human
- Clone
- 1C2
- Applications
- ELISA, WB, IF
- Conjugate
- Unconjugated
- Related Disease
- Noonan Syndrome 5 and Leopard Syndrome 2.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IF:1:20-1:200
Target
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Related Signaling Pathways
BCR Signaling Pathway
cAMP Signaling Pathway
ErbB Signaling Pathway
Insulin Signaling Pathway
MAPK Signaling Pathway
mTOR Signaling Pathway
PPAR Signaling Pathway
Ras Signaling Pathway
TCR Signaling Pathway
Related Diseases
Bladder Cancer
Breast Cancer
Colorectal Cancer
Endometrial Cancer
Gastric Cancer
Non-small Cell Lung Cancer
Prostate Cancer
Hepatocellular Carcinoma
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| MRO-2367-CN | Recombinant Rabbit Anti-RAF1 (phosphorylated Ser259) Monoclonal Antibody (CBACN-653) | WB, IHC | Rabbit IgG |
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| VS3-CJ324 | Rabbit Anti-RAF1 Recombinant Antibody (VS3-CJ324) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS-1024-XY138 | Mouse Anti-NHP RAF1 Recombinant Antibody (clone 4G4) | WB, IHC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS-0325-XY1866 | Anti-RAF1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5965 | Anti-Mouse RAF1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5964 | Anti-Human RAF1 Immunohistochemistry Kit | IHC | |
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