Rabbit Anti-Phospho-RAF1 (S621) Recombinant Antibody (clone 1C2) (CAT#: ZG-0539U)
Rabbit Anti-Phospho-RAF1 (S621) Antibody (clone 1C2) displayed high specificity against the RAF1 protein of Human.
Specific Inquiry
WB
Figure 1 Rabbit Anti-Phospho-RAF1 (S621) Antibody (ZG-0539U) in WB
Western Blot
Positive WB detected in Hela whole cell lysate(treated with Calyculin A or EGF)
All lanes Phospho-RAF1 antibody at 1.525μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 73 KDa
Observed band size: 73 KDa
IF
Figure 2 Rabbit Anti-Phospho-RAF1 (S621) Antibody (ZG-0539U) in IF
Immunofluorescence staining of HepG2 cells(treated with 50mM Calyculin A for 30min) with ZG-0539U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-RAF1 (S621)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Phospho-RAF1 (S621)
- Species Reactivity
- Human
- Clone
- 1C2
- Applications
- ELISA, WB, IF
- Conjugate
- Unconjugated
- Related Disease
- Noonan Syndrome 5 and Leopard Syndrome 2.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IF:1:20-1:200
Target
- Alternative Names
- RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1; RAF1; RAF
- Gene ID
- 5894
- UniProt ID
- P04049
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1388CT | Recombinant Mouse anti-Human RAF1 Monoclonal antibody (520) | WB | |
| MRO-1316-CN | Recombinant Rabbit Anti-RAF1 Monoclonal Antibody (CBACN-476) | WB, IF | Rabbit IgG |
| VS3-QX955 | Mouse Anti-RAF1 Recombinant Antibody (clone 4G4) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-FY1244 | Recombinant Rabbit Anti-RAF1 Antibody (clone R05-9B1) | WB, ICC, IF | Rabbit IgG |
| VS3-FY1245 | Recombinant Rabbit Anti-RAF1 (phospho Ser259) Antibody (clone R07-4J4) | WB, IHC-P | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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