Rabbit Anti-Phospho-SMAD2 (S255) Recombinant Antibody (clone 2A12) (CAT#: ZG-0567U)
Rabbit Anti-Phospho-SMAD2 (S255) Antibody (clone 2A12) displayed high specificity against the SMAD2 protein of Human.
Specific Inquiry
IF
Figure 1 Rabbit Anti-Phospho-SMAD2 (S255) Antibody (ZG-0567U) in IF
Immunofluorescence staining of A549 cells with ZG-0567U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-SMAD2 (S255)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Phospho-SMAD2 (S255)
- Species Reactivity
- Human
- Clone
- 2A12
- Applications
- ELISA, IF
- Conjugate
- Unconjugated
- Related Disease
- Congenital Heart Defects, Multiple Types, 8, With Or Without Heterotaxy and Loeys-Dietz Syndrome 6.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunofluorescence. Specific conditions should be optimized by the end user.
IF:1:20-1:200
Target
- Alternative Names
- Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD2; MADH2; MADR2
- Gene ID
- 4087
- UniProt ID
- Q15796
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4052z | Mouse Anti-SMAD2 Recombinant Antibody (clone 30G7) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| ZG-0566U | Rabbit Anti-Phospho-SMAD2 (S250) Recombinant Antibody (clone 4D12) | ELISA, WB | Rabbit IgG |
| VS3-QX1018 | Mouse Anti-SMAD2 Recombinant Antibody (clone 4D3) | WB, IP, IF, IHC | Mouse IgG1 |
| VS3-FY1357 | Recombinant Rabbit Anti-SMAD2 (phospho Ser250) Antibody (clone R02-0D1) | WB | Rabbit IgG |
| VS3-FY2873 | Rabbit Anti-SMAD2 Recombinant Antibody (VS3-FY2873) | WB, IHC, ICC, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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