Mouse Anti-phosphorylated Tau Recombinant Antibody (clone CP13)
CAT#: VS-0623-WK89
The antibody clone CP13 was produced by immunizing mouse with tau. It is specific for phosphorylated (p-)tau (serine 202). It can be used for detection of human tau in ELISA, WB, IHC and IF staining.
Published Data
Tested Data
IHC
Figure 1 Cell lysates and media transfected with iBs and scFvs were applied to paraffin-embedded sections of 10-month-old rTg4510 tau transgenic mice.
Cortical staining of neurofibrillary tangles was visualized with a biotinylated anti-c-myc secondary antibody. EGFP palys as a negative control.
ELISA
Figure 2 Cell lysates and media containing scFvs and iBs were subjected to direct ELISA.
Plates were coated with recombinant full-length tau, p-tau, or amyloid β as a control. Anti-c-myc HRP secondary antibody served as a detection.
FucnS
Figure 3 p-Tau-Specific iBs and scFvs Delay the Onset of Hindlimb Paralysis of Homozygous JNPL3 Mice.
JNPL3 mice for the survival cohort were intraspinally injected at P0 with pAAV2/8 (2 × 10e10 genomes) expressing PHF1, CP13, and Tau5 scFvs. Control mice were injected with rAAV-EGFP. All injected mice reached a terminal state (paralysis) within 12 months post-injection (n = 10-12). Median age of terminal state and relative increase of lifespan.
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WB
Figure 1 Mouse Anti-phosphorylated Tau Recombinant Antibody (VS-0623-WK89) in WB.
Western blot analysis of VS-0623-WK89 was performed by loading human pTau S202 protein-BSA.
VS-0623-WK89 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Mouse IgG (H+L).
DB
Figure 2 Mouse Anti-phosphorylated Tau Recombinant Antibody (VS-0623-WK89) in DB.
Dot Blot analysis of VS-0623-WK89 was performed by coating with human pTau S202 protein-BSA.
VS-0623-WK89 incubation concentration: 2 ng/μL.
HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000).
ELISA
Figure 3 Mouse Anti-phosphorylated Tau Recombinant Antibody (VS-0623-WK89) in ELISA.
ELISA analysis of VS-0623-WK89 was performed by coating with human pTau S202 protein-BSA. Then blocked with BSA and incubated with VS-0623-WK89. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Specifications
- Immunogen
- Tau
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human tau
- Species Reactivity
- Human
- Clone
- CP13
- Applications
- ELISA, WB, IHC, IF, FuncS
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No Preservative
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Immunohistochemical staining was done with cell lysates and media containing scFvs and iBs diluted 1/10 with PBS and applied to paraffin-fixed brain tissue slides from 10-month-old mice.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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