What specific form of Tau does this antibody recognize? I'm studying phosphorylation.

This antibody, clone CP13, is specifically for your line of research. It is designed to recognize the phosphorylated form of the Tau protein (MAPT) at the Serine 202 (Ser202) site. It is a highly specific mouse IgG1 monoclonal antibody and is a well-established tool for studying Tau phosphorylation.

need to detect human tau protein in tissue samples using IHC and also in cell lysates via Western Blot. Can you confirm if your CP13 clone (VS-0623-WK89) is a mouse monoclonal antibody and if it has been validated for both IHC and WB applications?

Thank you for your inquiry. We can confirm that our Anti-Tau Antibody, clone CP13, is indeed a mouse monoclonal antibody with an IgG1 isotype. It has been validated for specificity against human tau. The antibody is highly versatile and has been tested for a range of applications, including Western Blot (WB) and Immunohistochemistry (IHC), as well as ELISA, Immunofluorescence (IF), and Functional Studies (FuncS), making it well-suited for your research.

Mouse Anti-phosphorylated Tau Recombinant Antibody (clone CP13)

CAT#: VS-0623-WK89

The antibody clone CP13 was produced by immunizing mouse with tau. It is specific for phosphorylated (p-)tau (serine 202). It can be used for detection of human tau in ELISA, WB, IHC and IF staining.

Published Data Tested Data

IHC

Figure 1 Cell lysates and media transfected with iBs and scFvs were applied to paraffin-embedded sections of 10-month-old rTg4510 tau transgenic mice.

Cortical staining of neurofibrillary tangles was visualized with a biotinylated anti-c-myc secondary antibody. EGFP palys as a negative control.

ELISA

Figure 2 Cell lysates and media containing scFvs and iBs were subjected to direct ELISA.

Plates were coated with recombinant full-length tau, p-tau, or amyloid β as a control. Anti-c-myc HRP secondary antibody served as a detection.

FucnS

Figure 3 p-Tau-Specific iBs and scFvs Delay the Onset of Hindlimb Paralysis of Homozygous JNPL3 Mice.

JNPL3 mice for the survival cohort were intraspinally injected at P0 with pAAV2/8 (2 × 10e10 genomes) expressing PHF1, CP13, and Tau5 scFvs. Control mice were injected with rAAV-EGFP. All injected mice reached a terminal state (paralysis) within 12 months post-injection (n = 10-12). Median age of terminal state and relative increase of lifespan.

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Figure 1 Mouse Anti-phosphorylated Tau Recombinant Antibody (VS-0623-WK89) in WB.

Western blot analysis of VS-0623-WK89 was performed by loading human pTau S202 protein-BSA.
VS-0623-WK89 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Mouse IgG (H+L).

Figure 2 Mouse Anti-phosphorylated Tau Recombinant Antibody (VS-0623-WK89) in DB.

Dot Blot analysis of VS-0623-WK89 was performed by coating with human pTau S202 protein-BSA.
VS-0623-WK89 incubation concentration: 2 ng/μL.
HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000).

ELISA

Figure 3 Mouse Anti-phosphorylated Tau Recombinant Antibody (VS-0623-WK89) in ELISA.

ELISA analysis of VS-0623-WK89 was performed by coating with human pTau S202 protein-BSA. Then blocked with BSA and incubated with VS-0623-WK89. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

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Specifications

Immunogen
Tau

Host Species
Mouse

Type
Mouse IgG1

Specificity
Human tau

Species Reactivity
Human

Clone
CP13

Applications
ELISA, WB, IHC, IF, FuncS

Conjugate
Unconjugated

Product Property

Clonality
Monoclonal

Purification
Affinity purified

Purity
>95% as determined by SDS-PAGE

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No Preservative

Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
Immunohistochemical staining was done with cell lysates and media containing scFvs and iBs diluted 1/10 with PBS and applied to paraffin-fixed brain tissue slides from 10-month-old mice.

Target

Alternative Names
TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; tau-40; FTDP-17; PPP1R103; Tau-PHF6

Gene ID
4137

UniProt ID
P10636

Long Name
Microtubule associated protein tau

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Breast cancer biomarkers at key points during disease progression

Classic Phospho-Tau Clone

The CP13 clone is a standard for detecting Tau phosphorylated at Ser202, and this recombinant version is great. I used it for Immunohistochemistry (IHC) on mouse brain tissue, and it worked perfectly. The listed species reactivity (Human) makes it very suitable for neurobiology.

Specific for p-Tau (Ser202)

This antibody (VS-0623-WK89) is exactly what I needed. It's the mouse IgG1 clone CP13, which is highly specific for phosphorylated Tau at the Ser202 site. I'm using it for Western Blot (WB), which is one of the validated applications, and it gives a clean, specific band on human lysate.

Q&As

What specific form of Tau does this antibody recognize? I'm studying phosphorylation.

A: This antibody, clone CP13, is specifically for your line of research. It is designed to recognize the phosphorylated form of the Tau protein (MAPT) at the Serine 202 (Ser202) site. It is a highly specific mouse IgG1 monoclonal antibody and is a well-established tool for studying Tau phosphorylation.
need to detect human tau protein in tissue samples using IHC and also in cell lysates via Western Blot. Can you confirm if your CP13 clone (VS-0623-WK89) is a mouse monoclonal antibody and if it has been validated for both IHC and WB applications?

A: Thank you for your inquiry. We can confirm that our Anti-Tau Antibody, clone CP13, is indeed a mouse monoclonal antibody with an IgG1 isotype. It has been validated for specificity against human tau. The antibody is highly versatile and has been tested for a range of applications, including Western Blot (WB) and Immunohistochemistry (IHC), as well as ELISA, Immunofluorescence (IF), and Functional Studies (FuncS), making it well-suited for your research.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-0623-WK89.pdf

MSDS

MSDS - VS-0623-WK89.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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