Rabbit Anti-PIK3CA Recombinant Antibody (clone 10E5) (CAT#: ZG-0511U)
Rabbit Anti-PIK3CA Antibody (clone 10E5) displayed high specificity against the PIK3CA protein of Human and Mouse.
Specific Inquiry
WB
Figure 1 Rabbit Anti-PIK3CA Antibody (ZG-0511U) in WB
Western Blot
Positive WB detected in: Jurkat whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate
All lanes: PIK3CA antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 125 kDa
Observed band size: 110 kDa
IF
Figure 2 Rabbit Anti-PIK3CA Antibody (ZG-0511U) in IF
Immunofluorescence staining of Hela Cells with ZG-0511U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human PI 3 Kinase catalytic subunit alpha
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PIK3CA
- Species Reactivity
- Human, Mouse
- Clone
- 10E5
- Applications
- ELISA, WB, IF
- Conjugate
- Unconjugated
- Related Disease
- Hepatocellular Carcinoma and Megalencephaly-Capillary Malformation-Polymicrogyria Syndrome.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IF:1:20-1:200
Target
- Alternative Names
- Phosphatidylinositol 4;5-bisphosphate 3-kinase catalytic subunit alpha isoform; PI3-kinase subunit alpha; PI3K-alpha; PI3Kalpha; PtdIns-3-kinase subunit alpha; EC 2.7.1.153; Phosphatidylinositol 4;5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; PtdIns-3-kinase subunit p110-alpha; p110alpha; Phosphoinositide-3-kinase catalytic alpha polypeptide; Serine/threonine protein kinase PIK3CA; EC 2.7.11.1; PIK3CA
- Gene ID
- 5290
- UniProt ID
- P42336
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1459z | Mouse Anti-PIK3CA Recombinant Antibody (clone 20G7) | WB, IP, IF, FC, ELISA | Mouse IgG1, κ |
| MRO-1221-CN | Recombinant Rabbit Anti-PIK3CA Monoclonal Antibody (SJ0186) | WB, IF, IP | Rabbit IgG |
| VS3-CJ951 | Mouse Anti-PIK3CA Recombinant Antibody (clone ABT-PI3.H1047R) | WB, IF, IHC-P | Mouse IgG3, κ |
| VS3-CJ952 | Mouse Anti-PIK3CA Recombinant Antibody (clone PT0828) | WB, IHC-P | Mouse IgG1, κ |
| VS3-FY1138 | Recombinant Mouse Anti-PIK3CA Antibody (clone 3C1-6F9-6C4) | IHC-P | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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