Mouse Anti-PIK3CA Recombinant Antibody (clone ABT-PI3.H1047R) (CAT#: VS3-CJ951)

• Immunogen
• Synthesized peptide derived from human PIK3CA H1047R

• Host Species
• Mouse

• Type
• Mouse IgG3, κ

• Specificity
• Human PIK3CA

• Species Reactivity
• Human

• Clone
• ABT-PI3.H1047R

• Applications
• WB, IF, IHC-P

• Conjugate
• Unconjugated

• Purification
• Affinity Chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.5% BSA, PBS.

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Western Blot (1:500-1:2000), Immunofluorescence (1:500-1:200), Immunohistochemistry-Parraffin (1:100-1:500).

• Alternative Names
• MCM; CWS5; MCAP; PI3K; CLAPO; CLOVE; MCMTC; PI3K-alpha; p110-alpha

• Gene ID
• 5290

• UniProt ID
• P42336

• Sequence Similarities
• Belongs to the PI3/PI4-kinase family.

• Cellular Localization
• Cytoplasm, Cytosol, Intercalated disc, Perinuclear region of cytoplasm, Plasma membrane

• Protein Refseq
• NP_006209.2

• Function
• Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed:15135396, PubMed:23936502, PubMed:28676499).
  Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed:15135396, PubMed:28676499).
  PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. In addition to its lipid kinase activity, it displays a serine-protein kinase activity that results in the autophosphorylation of the p85alpha regulatory subunit as well as phosphorylation of other proteins such as 4EBP1, H-Ras, the IL-3 beta c receptor and possibly others (PubMed:23936502, PubMed:28676499).
  Plays a role in the positive regulation of phagocytosis and pinocytosis (By similarity).

Isotype Control

• MOB-205CQ
• Mouse IgG3, kappa Isotype Control Antibody

Secondary Antibody

• 2RD-A1470-YJ
• Goat Anti-Mouse IgG3 Secondary antibody

• 2RD-A1569-YJ
• Rabbit Anti-Mouse IgG3 Secondary antibody (Clone RM218)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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