Human Anti-PIV3 Prefusion Recombinant Antibody (PABJ-0161)

Customer Review

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Rachel Adams
25.Apr,23

Excellent for PIV3 Research

The Anti-PIV3 Prefusion Recombinant Antibody has been excellent for our PIV3 studies. Its quality is top-notch, providing clear and reliable results. This product has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.

Michael Clark
07.Aug,22

Effective and Dependable

We incorporated this antibody into our PIV3 protocols, and it has been a great asset. The consistency and reliability are impressive, allowing for accurate and reproducible results. The reliable performance has made it an invaluable tool in our lab.

Laura Harris
03.Jun,21

Consistent High-Quality Results

This antibody has proven to be consistent and high-quality in our PIV3 procedures. The reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The consistent performance has greatly enhanced our data quality.

Q&As

1. Is the anti-PIV3 prefusion recombinant antibody clone PIA174 suitable for use in Western blotting?

   A: Yes, the anti-PIV3 prefusion recombinant antibody clone PIA174 (PABJ-0161) is suitable for use in Western blotting. It provides specific binding to the PIV3 prefusion protein, allowing for reliable detection in Western blot assays.

2. What are the storage recommendations for the anti-PIV3 prefusion recombinant antibody clone PIA174?

   A: The recommended storage condition for the anti-PIV3 prefusion recombinant antibody clone PIA174 (PABJ-0161) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

3. Can the anti-PIV3 prefusion recombinant antibody clone PIA174 be used in immunoprecipitation assays?

   A: Yes, the anti-PIV3 prefusion recombinant antibody clone PIA174 (PABJ-0161) can be used in immunoprecipitation assays. It provides specific binding to the PIV3 prefusion protein, enabling the successful precipitation of the target protein from complex mixtures.

4. Is the anti-PIV3 prefusion recombinant antibody clone PIA174 effective in ELISA applications?

   A: Yes, the anti-PIV3 prefusion recombinant antibody clone PIA174 (PABJ-0161) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of the PIV3 prefusion protein.

5. What is the optimal dilution for using the anti-PIV3 prefusion recombinant antibody clone PIA174 in immunofluorescence?

   A: The optimal dilution for using the anti-PIV3 prefusion recombinant antibody clone PIA174 (PABJ-0161) in immunofluorescence is typically 1:100 to 1:500. It is advisable to perform a dilution series to determine the best working concentration for your specific experimental conditions.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

1. Marcink, Tara C., et al. "Subnanometer structure of an enveloped virus fusion complex on viral surface reveals new entry mechanisms." Science Advances 9.6 (2023): eade2727. https://doi.org/10.1126/sciadv.ade2727
   The study aims to investigate the structure of the fusion complex of human parainfluenza virus 3 (HPIV3) using cryo-electron tomography. It specifically focuses on the interaction between the hemagglutinin-neuraminidase (HN) and fusion (F) proteins, which are essential for viral entry into host cells. By visualizing these proteins at subnanometer resolution, the researchers were able to detail how HN and F proteins remain in a quiescent state on the viral surface until activation and how HN facilitates F's role in the fusion process. This research provides insights into the mechanisms of viral entry and potential targets for antiviral therapies.
   Creative Biolabs contributed to this research by supplying the PIA174 antibody Fab fragment (Cat# PABJ-0161-F(E)) and full antibody (Cat# PABJ-0161) used in the study. The catalog number for these products is not explicitly mentioned in the document. These antibodies were crucial for structural studies, allowing the researchers to stabilize and visualize the prefusion F complex and understand the precise interaction between HN and F proteins. The use of these antibodies facilitated the detailed examination of the viral fusion mechanism, which is essential for developing strategies to prevent viral entry into host cells.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# PABJ-0161, RRID: AB_3111610)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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