Mouse Anti-PKM Recombinant Antibody (clone 6C3C7) (CAT#: ZG-0247U)
Mouse anti-PKM antibody (clone 6C3C7) displayed high specificity against the PKM protein of Human, Rat, Mouse and Rabbit.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Mouse Anti-PKM Antibody (ZG-0247U) in WB
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate All lanes: PKM antibody at 1: 4000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 58 kDa
Observed band size: 58 KDa
Exposure time: 1min
IHC
Figure 2 Mouse Anti-PKM Antibody (ZG-0247U) in IHC
IHC image of ZG-0247U diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Mouse Anti-PKM Antibody (ZG-0247U) in IF
Immunofluorescence staining of A549 cells with ZG-0247U at 1:230, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).
FC
Figure 4 Mouse Anti-PKM Antibody (ZG-0247U) in FC
Overlay histogram showing Hela cells stained with ZG-0247U (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.
Specifications
- Immunogen
- Recombinant Human Pyruvate kinase PKM protein (2-531AA)
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PKM
- Species Reactivity
- Human, Rat, Mouse, Rabbit
- Clone
- 6C3C7
- Applications
- ELISA, WB, IHC, IF, FC, IP
- Conjugate
- Unconjugated
- Related Disease
- Pyruvate Kinase Deficiency Of Red Cells and Anterior Dislocation Of Lens
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation. Specific conditions should be optimized by the end user.
WB:1:4000-1:256000, IHC: 1:200-1:500, IF: 1:150-1:300, FC: 1:50-1:200, IP: 1µl-4µl
Target
- Alternative Names
- PK3; TCB; p58; OIP3; PKM2; CTHBP; THBP1; HEL-S-30; pyruvate kinase PKM; OPA-interacting protein 3; PK, muscle type; cytosolic thyroid hormone-binding protein; epididymis secretory protein Li 30; pyruvate kinase 2/3; pyruvate kinase isozymes M1/M2; pyruvate kinase muscle isozyme; pyruvate kinase, muscle; thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; tumor M2-PK
- Gene ID
- 5315
- UniProt ID
- P14618
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "PKM"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
Non-Human Primate (NHP) Antibody Products
Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0378C | Mouse Anti-PKM Recombinant Antibody (ZG-0378C) | WB, ICC | Mouse IgG |
| ZG-0379C | Mouse Anti-PKM Recombinant Antibody (ZG-0379C) | WB, ICC | Mouse IgG |
| ZG-0248U | Mouse Anti-PKM Recombinant Antibody (clone 4C8E12) | ELISA, WB, IHC, IF, FC, IP | Mouse IgG1 |
| ZG-0513U | Rabbit Anti-PKM Recombinant Antibody (clone 7B2) | ELISA, WB, IHC, IF, FC, IP | Rabbit IgG |
| VS3-XY3466 | Rabbit Anti-PKM Recombinant Antibody (clone R09-7E7) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for ZG-0247U. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
-2.png)
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: WB, IF, IP, Neut, FuncS, ELISA, FC
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: WB, IHC, ELISA, FC
Application: ELISA, WB
For Research Use Only. Not For Clinical Use.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
