Human Anti-Plasma cell malignancies Recombinant Antibody (clone chWue-1)
CAT#: HPAB-A1304-YJ
This product is a chimeric (mouse/human) antibody that recognizes Plasma cell malignancies. Wue-1 is Highly Specific for Healthy and Malignant Plasma Cells. Wue-1 did not react with macrophages or other lymphatic cells, such as T- lymphocytes or macrophages, and did not show any binding in the peripheral blood, liver tissue, kidneys, muscles, skin, urinary bladder, testis and ovary, and reacted weakly and inconstantly with secretory epithelium in the gastro intestinal duct (stomach, intestine).
Published Data
WB
Figure 1 Western blot analysis result of a plasma cell line (NC1-H929) with Wue-1/2.
FC
Figure 2 Expression of MV receptors and labelling with Wue-1 mAb.
(a-c) Two representative primary MM bone marrow samples (MM1 and MM2) and B cells from a healthy donor were stained with mAbs directed against Wue-1 (a), CD46 (b) and CD150 (SLAM) (c).
Hummel, H. D., Kuntz, G., Russell, S. J., Nakamura, T., Greiner, A., Einsele, H., & Topp, M. S. (2009). Genetically engineered attenuated measles virus specifically infects and kills primary multiple myeloma cells. Journal of general virology, 90(3), 693-701.
IHC
Figure 3 Primary bone marrow sample of an MM patient stained with mAb Wue-1, showing extensive labelling of the MM cells.
Hummel, H. D., Kuntz, G., Russell, S. J., Nakamura, T., Greiner, A., Einsele, H., & Topp, M. S. (2009). Genetically engineered attenuated measles virus specifically infects and kills primary multiple myeloma cells. Journal of general virology, 90(3), 693-701.
Inhib
Figure 4 Inhibition of MM cell infection by pre-treatment with Wue-1 mAb resulting in an approximate 60±10 % reduction in EGFP expression.
Hummel, H. D., Kuntz, G., Russell, S. J., Nakamura, T., Greiner, A., Einsele, H., & Topp, M. S. (2009). Genetically engineered attenuated measles virus specifically infects and kills primary multiple myeloma cells. Journal of general virology, 90(3), 693-701.
FuncS
Figure 5 MV-Wue was able to replicate as efficiently as its non-retargeted counterpart in primary MM cells, and cell entry was mediated by specific interaction of the virus with its target receptor.
Hummel, H. D., Kuntz, G., Russell, S. J., Nakamura, T., Greiner, A., Einsele, H., & Topp, M. S. (2009). Genetically engineered attenuated measles virus specifically infects and kills primary multiple myeloma cells. Journal of general virology, 90(3), 693-701.
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Specifications
- Host Species
- Human
- Derivation
- Chimeric (mouse/human)
- Type
- Chimeric (mouse/human) IgG1
- Specificity
- Human Plasma cell malignancies
- Species Reactivity
- Human
- Clone
- chWue-1
- Applications
- IHC, WB, FC, Inhib, FuncS
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the Certificate of Analysis for the lot-specific concentration.
- Buffer
- PBS
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Immunohistochemistry, Western Blot, Flow Cytometry, Inhibition, and Functional Assay. Optimal working dilutions must be determined by the end user.
Target
- Alternative Names
- plasmacytoma; myeloma
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-A1303-YJ | Mouse Anti-Plasma cell malignancies Recombinant Antibody (clone Wue-1) | IHC, WB, FC, Inhib, FuncS | Mouse IgG2a |
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