Mouse Anti-Plasmodium falciparum PFS230 Recombinant Antibody (clone 4F12) (CAT#: PABS-0220)

Figure 1 Epitope mapping of 4F12 by ELISA using the two proteins, Pf230D1M and Pfs230NS.

An additional form of Pfs230D1M, identified as Pfs230D1A was produced, which is homologous to Pfs230D1M except for amino acids 585QNT587, which were changed to 585NNA587.

Singh, K., Burkhardt, M., Nakuchima, S., Herrera, R., Muratova, O., Gittis, A. G., ... & Narum, D. L. (2020). Structure and function of a malaria transmission blocking vaccine targeting Pfs230 and Pfs230-Pfs48/45 proteins. Communications biology, 3(1), 1-12.

Figure 2 Pfs230, Pfs48/45, and Pfs230-Pfs48/45 subcellular localization in P. falciparum macrogametes and microgametes by confocal microscopy under live and/or fixed conditions.

A. Live emerged macrogametes were labeled with Pfs230D1-specific mAbs (mAb) as listed at the left. To demonstrate the presence of Pfs230, macrogametes were fixed and labeled with Pfs230D1-specific rabbit polyclonal (p) Abs (Rbα230). The third column shows the same cells by differential interference contrast (DIC) microscopy. 4F12 reacted with the surfaces of live macrogametes, but that 5G3, 5H1, and 7G4 did not. B. Live emerged macrogametes were labeled with pAbs (Rbα230) then fixed and stained with the same Pfs230D1-specific mAbs as panel A. After fixation all mAbs reacted with the surface of the macrogametes.

Singh, K., Burkhardt, M., Nakuchima, S., Herrera, R., Muratova, O., Gittis, A. G., ... & Narum, D. L. (2020). Structure and function of a malaria transmission blocking vaccine targeting Pfs230 and Pfs230-Pfs48/45 proteins. Communications biology, 3(1), 1-12.

Figure 3 Pfs230, Pfs48/45, and Pfs230-Pfs48/45 subcellular localization in P. falciparum macrogametes and microgametes by confocal microscopy under live and/or fixed conditions.

C. Live microgametes were labeled with Pfs230D1-specific mAbs as listed at left. To demonstrate the presence of Pfs230, they were fixed and labeled with Pfs230D1-specific pAbs (Rbα230). 4F12 and 5H1 recognized live microgametes, unlike 3G2. D. Fixed microgametes were labeled with pAbs (Rbα230) and with Pfs230D1-specific mAbs. After fixation, all mAbs recognize Pfs230. Only 4F12 recognized live macrogametes in A while both 4F12 and 5H1 recognized live microgametes in c.

Singh, K., Burkhardt, M., Nakuchima, S., Herrera, R., Muratova, O., Gittis, A. G., ... & Narum, D. L. (2020). Structure and function of a malaria transmission blocking vaccine targeting Pfs230 and Pfs230-Pfs48/45 proteins. Communications biology, 3(1), 1-12.

Figure 4 In both panels, 4F12 (circles) and 5H1 (triangles) were bound to ELISA plates.

On the left, Pfs230D1-specific mAb 4F12 was added as competitor, resulting in competition on the 4F12 plate (circles), but no competition on the 5H1 plate (triangles). On the right, is the analogous experiment, but with 5H1 as the competitor. 4F12 and 5H1 do not compete for binding to Pfs230D1 as seen by competitive ELISA. As a control in each panel, it can be seen that each mAb does compete against itself.

Singh, K., Burkhardt, M., Nakuchima, S., Herrera, R., Muratova, O., Gittis, A. G., ... & Narum, D. L. (2020). Structure and function of a malaria transmission blocking vaccine targeting Pfs230 and Pfs230-Pfs48/45 proteins. Communications biology, 3(1), 1-12.

Figure 5 Combining Pfs230D1-specific mAbs, 4F12 and 5H1, show enhanced TR activity (“mAbs combined”) compared to each set of mAbs alone.

The average inhibition of oocyst density was 40.3%, 86.0%, and 24.8% against the control (0%) at IgG concentrations: 1.1, 1.1 + 0.63, 0.63, and 1.0 mg/mL, respectively.

Singh, K., Burkhardt, M., Nakuchima, S., Herrera, R., Muratova, O., Gittis, A. G., ... & Narum, D. L. (2020). Structure and function of a malaria transmission blocking vaccine targeting Pfs230 and Pfs230-Pfs48/45 proteins. Communications biology, 3(1), 1-12.

Figure 6 Combining Pfs230D1-specific mAb rh4F12 (“rh4F12_Pfs230”) and Pfs48/45-specific mAb 3E12 (“3E12_Pfs48/45”) shows enhanced TB activity (“mAbs combined”) compared to each set of mAbs alone.

The average inhibition of oocyst density was 92.3%, 99.4%, and 82.6% against the control (0%) at IgG concentrations: 0.63, 0.63 + 0.04, 0.04, and 0.94 mg/mL, respectively.

Singh, K., Burkhardt, M., Nakuchima, S., Herrera, R., Muratova, O., Gittis, A. G., ... & Narum, D. L. (2020). Structure and function of a malaria transmission blocking vaccine targeting Pfs230 and Pfs230-Pfs48/45 proteins. Communications biology, 3(1), 1-12.