Mouse Anti-PLIN3 Recombinant Antibody (VS3-CJ156) (CAT#: VS3-CJ156)

• Immunogen
• KLH-conjugated synthetic peptide between 1-434 amino acids from human PLIN3

• Host Species
• Mouse

• Type
• Mouse IgG2a, κ

• Specificity
• Human PLIN3

• Species Reactivity
• Human

• Applications
• WB

• Conjugate
• Unconjugated

• Purification
• Affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• PBS

• Preservative
• 0.09% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Western Blot (1:2000), Flow Cytometry (1:25).

• Alternative Names
• Perilipin-3; 47 kDa mannose 6-phosphate receptor-binding protein; 47 kDa MPR-binding protein; Cargo selection protein TIP47; Mannose-6-phosphate receptor-binding protein 1; Placental protein 17; PP17; PLIN3; M6PRBP1; TIP47

• Gene ID
• 10226

• UniProt ID
• O60664

• Sequence Similarities
• Belongs to the perilipin family.

• Cellular Localization
• Cytoplasm, Endosome, Lipid droplet, Membrane

• Post Translation Modifications
• Phosphorylation at Tyr-251 by isoform 1 of CHKA (CHKalpha2) promotes dissociation from lipid droplets: dissociation is followed by recruitment of autophagosome machinery to lipid droplets and subsequent lipid droplet lipolysis.

• Protein Refseq
• NP_001157661.1; NP_001157666.1; NP_005808.3

• Function
• Structural component of lipid droplets, which is required for the formation and maintenance of lipid storage droplets (PubMed:34077757).
  Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network (PubMed:9590177).

Isotype Control

• MOB-202CQ
• Mouse IgG2a, kappa Isotype Control Antibody

Secondary Antibody

• 2RD-A1565-YJ
• Rabbit Anti-Mouse IgG2a Kappa Secondary antibody (Clone RM107)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS