Mouse Anti-PODXL Monoclonal Antibody (VS7-0425-WR644) (CAT#: VS7-0425-WR644)

• Immunogen
• Recombinant human PODXL fragment expressed in E. Coli.

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human PODXL

• Species Reactivity
• Human

• Molecule Class
• Transmembrane Sialomucin Glycoprotein

• Applications
• WB, IHC-P, IF, FC, ELISA

• Conjugate
• Unconjugated

• Clonality
• Monoclonal

• Purity
• >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid

• Format
• Liquid

• Concentration
• 1 mg/ml

• Preservative
• 0.03% sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• Western Blot: 1:500 - 1:2000
  Immunohistochemistry: 1:200 - 1:1000
  Flow Cytometry: 1:200 - 1:400
  Enzyme-linked Immunosorbent Assay: 1:10000

• Alternative Names
• PODXL; Podocalyxin-Like; PC; PCLP; Podocalyxin; GCTM-2 Antigen; PCLP-1; Gp200; PCLP1; Podocalyxin-Like Protein 1

• Gene ID
• 5420

• UniProt ID
• O00592

• Long Name
• Podocalyxin Like

• Cellular Localization
• Cell membrane; Cell projection; Membrane

• Post Translation Modifications
• Glycoprotein; Phosphoprotein

• Protein Refseq
• NP_001018121.1

• Function
• Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody

Select a product category from the dropdown menu below to view related products.