Mouse Anti-PODXL Monoclonal Antibody (VS7-0425-WR644) (CAT#: VS7-0425-WR644)
This product is a mouse IgG antibody that binds to human PODXL. The antibody can be used for WB, IHC-P, IF, FC, and ELISA assays.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli, plasma membrane, centriolar satellites & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2110/118_H1_2_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli, plasma membrane, centriolar satellites & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2110/118_H1_2_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli, plasma membrane & centriolar satellites.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2110/119_H1_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli, plasma membrane & centriolar satellites.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2110/119_H1_1_blue_red_green.jpg
Figure 3 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_B_9_5.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_B_9_5.jpg
Figure 4 Kidney
(Bowman's capsule
Staining:High
Intensity: Strong
Quantity:>75%
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_A_8_5.jpg
(Bowman's capsule
Staining:High
Intensity: Strong
Quantity:>75%
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_A_8_5.jpg
Figure 5 Endometrium
(Glandular cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_B_5_3.jpg
(Glandular cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_B_5_3.jpg
Figure 6 RNA cell line category: Cell line enhanced (fHDF/TERT166, NTERA-2, SuSa, TIME, U-251 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000128567-PODXL
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000128567-PODXL
Specifications
- Immunogen
- Recombinant human PODXL fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PODXL
- Species Reactivity
- Human
- Molecule Class
- Transmembrane Sialomucin Glycoprotein
- Applications
- WB, IHC-P, IF, FC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PODXL; Podocalyxin-Like; PC; PCLP; Podocalyxin; GCTM-2 Antigen; PCLP-1; Gp200; PCLP1; Podocalyxin-Like Protein 1
- Gene ID
- 5420
- UniProt ID
- O00592
- Long Name
- Podocalyxin Like
- Cellular Localization
- Cell membrane; Cell projection; Membrane
- Post Translation Modifications
- Glycoprotein; Phosphoprotein
- Protein Refseq
- NP_001018121.1
- Function
- Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1954-sdAb | Recombinant Anti-Human PODXL VHH Single Domain Antibody | IHC, ICC, FC, FA, FUNC | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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