Mouse Anti-PODXL Monoclonal Antibody (VS7-0425-WR645)
CAT#: VS7-0425-WR645
This product is a mouse IgG antibody that binds to human PODXL. The antibody can be used for WB and ELISA assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli, plasma membrane, centriolar satellites & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2110/118_H1_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli, plasma membrane & centriolar satellites.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2110/119_H1_1_blue_red_green.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_B_9_5.jpg
Normal Tissue
Figure 4 Kidney
Bowman's capsule
Staining:High
Intensity: Strong
Quantity:>75%
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_A_8_5.jpg
Normal Tissue
Figure 5 Endometrium
Glandular cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_B_5_3.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (fHDF/TERT166, NTERA-2, SuSa, TIME, U-251 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000128567-PODXL
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Specifications
- Immunogen
- Recombinant human PODXL fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PODXL
- Species Reactivity
- Human
- Molecule Class
- Transmembrane Sialomucin Glycoprotein
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PODXL; Podocalyxin-Like; PC; PCLP; Podocalyxin; GCTM-2 Antigen; PCLP-1; Gp200; PCLP1; Podocalyxin-Like Protein 1
- Gene ID
- 5420
- UniProt ID
- O00592
- Long Name
- Podocalyxin Like
- Cellular Localization
- Cell membrane; Cell projection; Membrane
- Post Translation Modifications
- Glycoprotein; Phosphoprotein
- Protein Refseq
- NP_001018121.1
- Function
- Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PODXL"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1954-sdAb | Recombinant Anti-Human PODXL VHH Single Domain Antibody | IHC, ICC, FC, FA, FUNC | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1056CL | Mouse Anti-PODXL Recombinant Antibody (TAB-1056CL) | ELISA, FC, Cyt, IP, WB, IHC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1053CL-S(P) | Rabbit Anti-PODXL Recombinant Antibody; scFv Fragment (TAB-1053CL-S(P)) | FC | Rabbit scFv |
| TAB-1054CL-S(P) | Mouse Anti-PODXL Recombinant Antibody; scFv Fragment (TAB-1054CL-S(P)) | FC | Mouse scFv |
| TAB-1053CL-F(E) | Rabbit Anti-PODXL Recombinant Antibody; Fab Fragment (TAB-1053CL-F(E)) | FC | Rabbit Fab |
| TAB-1054CL-F(E) | Mouse Anti-PODXL Recombinant Antibody; Fab Fragment (TAB-1054CL-F(E)) | FC | Mouse Fab |
| TAB-1056CL-F(E) | Mouse Anti-PODXL Recombinant Antibody; Fab Fragment (TAB-1056CL-F(E)) | IHC, FC, WB, ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2772 | Hi-Affi™ Recombinant Rabbit Anti-PODXL Monoclonal Antibody (DS2772AB) | WB, IHC-P, ICC, IF, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1253-CN | Recombinant Mouse Anti-PODXL Monoclonal Antibody (D0-E8) | WB, IHC | Mouse IgG1 |
| MRO-1254-CN | Recombinant Mouse Anti-PODXL Monoclonal Antibody (2-A4) | IF, IHC | Mouse IgG1 |
| HPAB-M0611-YC | Mouse Anti-PODXL Recombinant Antibody (HPAB-M0611-YC) | FC, IHC | Mouse IgG |
| HPAB-M0612-YC | Human Anti-PODXL Recombinant Antibody (HPAB-M0612-YC) | FC, IHC | Chimeric (Mouse/Human) IgG1 |
| VS3-XY3471 | Rabbit Anti-PODXL Recombinant Antibody (clone R01-6W4) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0611-YC-S(P) | Mouse Anti-PODXL Recombinant Antibody; scFv Fragment (HPAB-M0611-YC-S(P)) | FC, IHC | Mouse scFv |
| HPAB-M0612-YC-S(P) | Human Anti-PODXL Recombinant Antibody; scFv Fragment (HPAB-M0612-YC-S(P)) | FC, IHC | Human scFv |
| HPAB-3334LY-S(P) | Human Anti-PODXL Recombinant Antibody; scFv Fragment (HPAB-3334LY-S(P)) | FC, IHC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0611-YC-F(E) | Mouse Anti-PODXL Recombinant Antibody; Fab Fragment (HPAB-M0611-YC-F(E)) | FC, IHC | Mouse Fab |
| HPAB-M0612-YC-F(E) | Human Anti-PODXL Recombinant Antibody; Fab Fragment (HPAB-M0612-YC-F(E)) | FC, IHC | Chimeric (Mouse/Human) Fab |
| HPAB-3334LY-F(E) | Human Anti-PODXL Recombinant Antibody; Fab Fragment (HPAB-3334LY-F(E)) | FC, IHC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC271 | Recombinant Anti-PODXL Vesicular Antibody, EV Displayed (VS-0425-YC271) | Cyt, ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5535 | Anti-Mouse PODXL Immunohistochemistry Kit | IHC | |
| VS-0525-XY5536 | Anti-Rat PODXL Immunohistochemistry Kit | IHC | |
| VS-0525-XY5537 | Anti-Rabbit PODXL Immunohistochemistry Kit | IHC |
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