Rabbit Anti-PODXL Recombinant Antibody (clone R01-6W4) (CAT#: VS3-XY3471)
It is a recombinant rabbit antibody targeting the PODXL (Podocalyxin like), which involves in the regulation of both adhesion and cell morphology and cancer progression. PODXL also induces the formation of apical actin-dependent microvilli. The antibody specifically recognizes and binds to human PODXL. It has been determined using Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli, plasma membrane, centriolar satellites & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2110/118_H1_2_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli, plasma membrane, centriolar satellites & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2110/118_H1_2_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli, plasma membrane & centriolar satellites.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2110/119_H1_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli, plasma membrane & centriolar satellites.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2110/119_H1_1_blue_red_green.jpg
Figure 3 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_B_9_5.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_B_9_5.jpg
Figure 4 Kidney
(Bowman's capsule
Staining:High
Intensity: Strong
Quantity:>75%
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_A_8_5.jpg
(Bowman's capsule
Staining:High
Intensity: Strong
Quantity:>75%
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_A_8_5.jpg
Figure 5 Endometrium
(Glandular cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_B_5_3.jpg
(Glandular cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_B_5_3.jpg
Figure 6 RNA cell line category: Cell line enhanced (fHDF/TERT166, NTERA-2, SuSa, TIME, U-251 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000128567-PODXL
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000128567-PODXL
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PODXL
- Species Reactivity
- Human
- Clone
- R01-6W4
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Related Disease
- Atypical Juvenile Parkinsonism; Young-Onset Parkinson Disease
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- PC; PDX; PCLP; Gp200; gp135; PCLP-1; PODXL1
- Gene ID
- 5420
- UniProt ID
- O00592
- Long Name
- Podocalyxin like
- Cellular Localization
- Apical cell membrane, Cell projection, Lamellipodium
- Post Translation Modifications
- Ubiquitination at Lys526 (NX_O00592 [NX_O00592-1])
Modification sites at PhosphoSitePlus (O00592)
Glycosylation from GlyConnect PODXL_HUMAN (1617)
Glycosylation from GlyGen (O00592) 66 sites, 21 N-linked glycans (2 sites), 9 O-linked glycans (60 sites)
- Protein Refseq
- NP_005388.2
- Function
- Involved in the regulation of both adhesion and cell morphology and cancer progression.
Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion.
Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner.
Induces the formation of apical actin-dependent microvilli.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1954-sdAb | Recombinant Anti-Human PODXL VHH Single Domain Antibody | IHC, ICC, FC, FA, FUNC | Llama VHH |
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For Research Use Only. Not For Clinical Use.
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