Rabbit Anti-PODXL Recombinant Antibody (clone R01-6W4)
CAT#: VS3-XY3471
It is a recombinant rabbit antibody targeting the PODXL (Podocalyxin like), which involves in the regulation of both adhesion and cell morphology and cancer progression. PODXL also induces the formation of apical actin-dependent microvilli. The antibody specifically recognizes and binds to human PODXL. It has been determined using Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli, plasma membrane, centriolar satellites & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2110/118_H1_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli, plasma membrane & centriolar satellites.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2110/119_H1_1_blue_red_green.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_B_9_5.jpg
Normal Tissue
Figure 4 Kidney
Bowman's capsule
Staining:High
Intensity: Strong
Quantity:>75%
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_A_8_5.jpg
Normal Tissue
Figure 5 Endometrium
Glandular cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_B_5_3.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (fHDF/TERT166, NTERA-2, SuSa, TIME, U-251 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000128567-PODXL
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PODXL
- Species Reactivity
- Human
- Clone
- R01-6W4
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Related Disease
- Atypical Juvenile Parkinsonism; Young-Onset Parkinson Disease
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- PC; PDX; PCLP; Gp200; gp135; PCLP-1; PODXL1
- Gene ID
- 5420
- UniProt ID
- O00592
- Long Name
- Podocalyxin like
- Cellular Localization
- Apical cell membrane, Cell projection, Lamellipodium
- Post Translation Modifications
- Ubiquitination at Lys526 (NX_O00592 [NX_O00592-1])
Modification sites at PhosphoSitePlus (O00592)
Glycosylation from GlyConnect PODXL_HUMAN (1617)
Glycosylation from GlyGen (O00592) 66 sites, 21 N-linked glycans (2 sites), 9 O-linked glycans (60 sites)
- Protein Refseq
- NP_005388.2
- Function
- Involved in the regulation of both adhesion and cell morphology and cancer progression.
Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion.
Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner.
Induces the formation of apical actin-dependent microvilli.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PODXL"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1954-sdAb | Recombinant Anti-Human PODXL VHH Single Domain Antibody | IHC, ICC, FC, FA, FUNC | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1053CL | Rabbit Anti-PODXL Recombinant Antibody (TAB-1053CL) | FC, FuncS | Rabbit antibody |
| TAB-1054CL | Mouse Anti-PODXL Recombinant Antibody (TAB-1054CL) | FC, FuncS | Mouse IgG |
| TAB-1053CL-S(P) | Rabbit Anti-PODXL Recombinant Antibody; scFv Fragment (TAB-1053CL-S(P)) | FC | Rabbit scFv |
| TAB-1054CL-S(P) | Mouse Anti-PODXL Recombinant Antibody; scFv Fragment (TAB-1054CL-S(P)) | FC | Mouse scFv |
| TAB-1056CL-S(P) | Mouse Anti-PODXL Recombinant Antibody; scFv Fragment (TAB-1056CL-S(P)) | IHC, FC, WB, ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1056CL | Mouse Anti-PODXL Recombinant Antibody (TAB-1056CL) | ELISA, FC, Cyt, IP, WB, IHC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1821MZ | Recombinant Mouse Anti-Human PODXL Antibody (clone USB-2-92) | FC, ICC, IF, IHC-Fr, IP, RIA, WB | Mouse antibody |
| HPAB-3334LY | Human Anti-PODXL Recombinant Antibody (HPAB-3334LY) | FC, IHC | Humanized IgG |
| ZG-0167U | Mouse Anti-PODXL Recombinant Antibody (clone 4D2G7) | ELISA, WB, IHC, IP, IF, FC | Mouse IgG2b |
| VS7-0425-WR644 | Mouse Anti-PODXL Monoclonal Antibody (VS7-0425-WR644) | WB, IHC-P, IF, FC, ELISA | Mouse IgG |
| VS7-0425-WR645 | Mouse Anti-PODXL Monoclonal Antibody (VS7-0425-WR645) | WB, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2772 | Hi-Affi™ Recombinant Rabbit Anti-PODXL Monoclonal Antibody (DS2772AB) | WB, IHC-P, ICC, IF, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0611-YC-S(P) | Mouse Anti-PODXL Recombinant Antibody; scFv Fragment (HPAB-M0611-YC-S(P)) | FC, IHC | Mouse scFv |
| HPAB-M0612-YC-S(P) | Human Anti-PODXL Recombinant Antibody; scFv Fragment (HPAB-M0612-YC-S(P)) | FC, IHC | Human scFv |
| HPAB-3334LY-S(P) | Human Anti-PODXL Recombinant Antibody; scFv Fragment (HPAB-3334LY-S(P)) | FC, IHC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0611-YC-F(E) | Mouse Anti-PODXL Recombinant Antibody; Fab Fragment (HPAB-M0611-YC-F(E)) | FC, IHC | Mouse Fab |
| HPAB-M0612-YC-F(E) | Human Anti-PODXL Recombinant Antibody; Fab Fragment (HPAB-M0612-YC-F(E)) | FC, IHC | Chimeric (Mouse/Human) Fab |
| HPAB-3334LY-F(E) | Human Anti-PODXL Recombinant Antibody; Fab Fragment (HPAB-3334LY-F(E)) | FC, IHC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC271 | Recombinant Anti-PODXL Vesicular Antibody, EV Displayed (VS-0425-YC271) | Cyt, ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5535 | Anti-Mouse PODXL Immunohistochemistry Kit | IHC | |
| VS-0525-XY5536 | Anti-Rat PODXL Immunohistochemistry Kit | IHC | |
| VS-0525-XY5537 | Anti-Rabbit PODXL Immunohistochemistry Kit | IHC |
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