Rabbit Anti-PODXL Recombinant Antibody (clone R01-6W4) (CAT#: VS3-XY3471)
It is a recombinant rabbit antibody targeting the PODXL (Podocalyxin like), which involves in the regulation of both adhesion and cell morphology and cancer progression. PODXL also induces the formation of apical actin-dependent microvilli. The antibody specifically recognizes and binds to human PODXL. It has been determined using Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli, plasma membrane, centriolar satellites & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2110/118_H1_2_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli, plasma membrane, centriolar satellites & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2110/118_H1_2_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli, plasma membrane & centriolar satellites.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2110/119_H1_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli, plasma membrane & centriolar satellites.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2110/119_H1_1_blue_red_green.jpg
Figure 3 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_B_9_5.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_B_9_5.jpg
Figure 4 Kidney
(Bowman's capsule
Staining:High
Intensity: Strong
Quantity:>75%
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_A_8_5.jpg
(Bowman's capsule
Staining:High
Intensity: Strong
Quantity:>75%
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_A_8_5.jpg
Figure 5 Endometrium
(Glandular cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_B_5_3.jpg
(Glandular cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62558/140923_B_5_3.jpg
Figure 6 RNA cell line category: Cell line enhanced (fHDF/TERT166, NTERA-2, SuSa, TIME, U-251 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000128567-PODXL
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000128567-PODXL
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PODXL
- Species Reactivity
- Human
- Clone
- R01-6W4
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Related Disease
- Atypical Juvenile Parkinsonism; Young-Onset Parkinson Disease
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- PC; PDX; PCLP; Gp200; gp135; PCLP-1; PODXL1
- Gene ID
- 5420
- UniProt ID
- O00592
- Long Name
- Podocalyxin like
- Cellular Localization
- Apical cell membrane, Cell projection, Lamellipodium
- Post Translation Modifications
- Ubiquitination at Lys526 (NX_O00592 [NX_O00592-1])
Modification sites at PhosphoSitePlus (O00592)
Glycosylation from GlyConnect PODXL_HUMAN (1617)
Glycosylation from GlyGen (O00592) 66 sites, 21 N-linked glycans (2 sites), 9 O-linked glycans (60 sites)
- Protein Refseq
- NP_005388.2
- Function
- Involved in the regulation of both adhesion and cell morphology and cancer progression.
Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion.
Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner.
Induces the formation of apical actin-dependent microvilli.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "PODXL"
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1954-sdAb | Recombinant Anti-Human PODXL VHH Single Domain Antibody | IHC, ICC, FC, FA, FUNC | Llama VHH |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1053CL | Rabbit Anti-PODXL Recombinant Antibody (TAB-1053CL) | FC, FuncS | Rabbit antibody |
| TAB-1054CL | Mouse Anti-PODXL Recombinant Antibody (TAB-1054CL) | FC, FuncS | Mouse IgG |
| TAB-1053CL-F(E) | Rabbit Anti-PODXL Recombinant Antibody; Fab Fragment (TAB-1053CL-F(E)) | FC | Rabbit Fab |
| TAB-1054CL-F(E) | Mouse Anti-PODXL Recombinant Antibody; Fab Fragment (TAB-1054CL-F(E)) | FC | Mouse Fab |
| TAB-1056CL-F(E) | Mouse Anti-PODXL Recombinant Antibody; Fab Fragment (TAB-1056CL-F(E)) | IHC, FC, WB, ELISA | Mouse Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1821MZ | Recombinant Mouse Anti-Human PODXL Antibody (clone USB-2-92) | FC, ICC/IF, IHC-Fr, IP, RIA, WB | Mouse antibody |
| HPAB-M0611-YC | Mouse Anti-PODXL Recombinant Antibody (HPAB-M0611-YC) | FC, IHC | Mouse IgG |
| HPAB-M0612-YC | Human Anti-PODXL Recombinant Antibody (HPAB-M0612-YC) | FC, IHC | Chimeric (Mouse/Human) IgG1 |
| VS3-QX891 | Mouse Anti-PODXL Recombinant Antibody (clone 632CT5.11.4) | WB, ICC | Mouse IgG1 |
| VS3-QX892 | Mouse Anti-PODXL Recombinant Antibody (clone 5F5) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2772 | Hi-Affi™ Recombinant Rabbit Anti-PODXL Monoclonal Antibody (DS2772AB) | WB, IHC-P, ICC/IF, IP | IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0611-YC-S(P) | Mouse Anti-PODXL Recombinant Antibody; scFv Fragment (HPAB-M0611-YC-S(P)) | FC, IHC | Mouse scFv |
| HPAB-M0612-YC-S(P) | Human Anti-PODXL Recombinant Antibody; scFv Fragment (HPAB-M0612-YC-S(P)) | FC, IHC | Human scFv |
| HPAB-3334LY-S(P) | Human Anti-PODXL Recombinant Antibody; scFv Fragment (HPAB-3334LY-S(P)) | FC, IHC | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0611-YC-F(E) | Mouse Anti-PODXL Recombinant Antibody; Fab Fragment (HPAB-M0611-YC-F(E)) | FC, IHC | Mouse Fab |
| HPAB-M0612-YC-F(E) | Human Anti-PODXL Recombinant Antibody; Fab Fragment (HPAB-M0612-YC-F(E)) | FC, IHC | Chimeric (Mouse/Human) Fab |
| HPAB-3334LY-F(E) | Human Anti-PODXL Recombinant Antibody; Fab Fragment (HPAB-3334LY-F(E)) | FC, IHC | Humanized Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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