Anti-POLR3D Immunohistochemistry Kit (CAT#: VS-0325-XY1698)
Our POLR3D IHC Kit features all the necessary reagents, simplifying the experiment preparation. It is engineered to be user - friendly, reducing the complexity of the procedure. This kit works well with both paraffin and frozen tissue sections, enabling reliable POLR3D detection in various tissue samples for research on non - coding RNA transcription.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61331/1101_C9_4_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61331/1101_C9_4_selected.jpg
Figure 2 IHC staining of human oral mucosa
(Immunohistochemical staining of human oral mucosa shows moderate nuclear positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67875/154832_A_7_1_selected.jpg
(Immunohistochemical staining of human oral mucosa shows moderate nuclear positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67875/154832_A_7_1_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67875/154832_B_9_5.jpg
(Endothelial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67875/154832_B_9_5.jpg
Figure 4 Esophagus
(Squamous epithelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68461/154848_B_5_1.jpg
(Squamous epithelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68461/154848_B_5_1.jpg
Figure 5 Colon
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68461/154848_A_9_3.jpg
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68461/154848_A_9_3.jpg
Figure 6 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68461/154848_A_7_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68461/154848_A_7_5.jpg
Figure 7 Testis
(Sertoli cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Spermatogonia cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68461/154848_A_6_6.jpg
(Sertoli cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Spermatogonia cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68461/154848_A_6_6.jpg
Figure 8 Lymph node
(Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68461/154848_A_7_8.jpg
(Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68461/154848_A_7_8.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168495-POLR3D/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168495-POLR3D/subcellular
Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human
- Target
- POLR3D
- Primary Antibody
- Rabbit Anti-POLR3D Antibody
- Secondary Antibody
- Goat anti-Rabbit Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3476z | Mouse Anti-POLR3D Recombinant Antibody (clone 19G10) | ELISA | Mouse IgG2a, κ |
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