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    Rabbit Anti-POMC Recombinant Antibody (clone R02-2J8) (CAT#: VS3-XY3472)

    This antibody is a recombinant rabbit antibody targeting the POMC (Proopiomelanocortin), which stimulates the adrenal glands to release cortisol. This antibody specifically reacts with human, mouse and rat POMC. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin and Immunoprecipitation. The recommended dilutions of Western blot, Immunohistochemistry-Paraffin and Immunoprecipitation is 1/500-1/1000, 1/50-1/100 and 1/20, respectively.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IHC staining of human pituitary gland
    Figure 1 IHC staining of human pituitary gland
    (Immunohistochemical staining shows high expression in pituitary gland)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/ih_additional_tissue/46135/849_266_200x200.jpg
    Normal Tissue
    Figure 2 Liver
    Figure 2 Liver
    (Hepatocytes
    Staining: Low
    Intensity: Moderate
    Quantity: <25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/46135/146483_A_8_4.jpg
    Figure 3 Testis
    Figure 3 Testis
    (Leydig cells
    Staining: Low
    Intensity: Weak
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/46135/146483_A_6_6.jpg
    RNA Expression
    Figure 4 RNA cell line category: Cell line enhanced (HMC-1, THP-1)
    Figure 4 RNA cell line category: Cell line enhanced (HMC-1, THP-1)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115138-POMC/subcellular

    Specifications

    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human, Mouse and Rat POMC
    • Species Reactivity
    • Human, Mouse, Rat
    • Clone
    • R02-2J8
    • Applications
    • WB, IHC-P, IP
    • Related Disease
    • Obesity, Early-Onset, With Adrenal Insufficiency

    Product Property

    • Clonality
    • Monoclonal
    • Aggregation
    • < 5%
    • Format
    • Liquid
    • Buffer
    • PBS (pH 7.4), 150 mM NaCl and 50% glycerol
    • Preservative
    • 0.02% sodium azide
    • Storage
    • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • WB: 1/500-1/1000
      IHC: 1/50-1/100
      IP: 1/20

    Target

    • Alternative Names
    • LPH; MSH; NPP; POC; ACTH; CLIP; OBAIRH
    • Long Name
    • Proopiomelanocortin
    • Cellular Localization
    • Secreted
    • Post Translation Modifications
    • Specific enzymatic cleavages at paired basic residues yield the different active peptides. (P01189-COLI_HUMAN)
      O-glycosylated; reducing sugar is probably N-acetylgalactosamine. (P01189-COLI_HUMAN)
      Glycosylation at Thr71 and Asn91 (NX_P01189 [NX_P01189-1])
      Modification sites at PhosphoSitePlus (P01189)
      Glycosylation from GlyGen (P01189) 2 sites
    • Function
    • [Corticotropin]: Stimulates the adrenal glands to release cortisol.
      [Melanocyte-stimulating hormone alpha]: Anorexigenic peptide.
      Increases the pigmentation of skin by increasing melanin production in melanocytes.

    Recommended Products

    Isotype Control

    Recommended Dilution Buffer

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "POMC"

    Select a product category from the dropdown menu below to view related products.
    Please select product type
    IgG Antibody Products Chicken IgY Antibody Products Rabbit Monoclonal Antibody Products
    CAT Product Name Application Type
    MOB-1827z Recombinant Anti-Human POMC Antibody ELISA, IF, FuncS IgG
    MOB-0258MZ Recombinant Mouse Anti-Human POMC Antibody (clone 03B4) IHC Mouse antibody
    ZG-0017J Mouse Anti-POMC Recombinant Antibody (ZG-0017J) IHC-P, IF Mouse IgG
    ZG-462C Mouse Anti-POMC Recombinant Antibody (clone 2F6H11) ELISA, IHC, IHC-Fr, IHC-P Mouse IgG1, κ
    VS3-FY32 Recombinant Mouse Anti-POMC Antibody (clone 3A4H4) ELISA, ICC, FC Mouse IgG1

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