Rabbit Anti-PPIA Recombinant Antibody (clone 4G3)
CAT#: ZG-0517U
Rabbit Anti-PPIA Antibody (clone 4G3) displayed high specificity against the PPIA protein of Human.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-PPIA Antibody (ZG-0517U) in WB
Western Blot
Positive WB detected in: Raji whole cell lysate, HepG2 whole cell lysate
All lanes: Cyclophilin A antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 19, 12 kDa
Observed band size: 19 kDa
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Subcellular Location and Protein Expression
Figure 1 IHC staining of human kidney
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58345/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4655/15600_B_8_5.jpg
Normal Tissue
Figure 3 Rectum
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4655/15600_A_4_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4655/15600_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4655/15600_A_5_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4655/15600_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196262-PPIA/subcellular
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Specifications
- Immunogen
- A synthesized peptide derived from human Cyclophilin A
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PPIA
- Species Reactivity
- Human
- Clone
- 4G3
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
- Related Disease
- Influenza and Measles.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot. Specific conditions should be optimized by the end user.
WB:1:500-1:5000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "PPIA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2166z | Mouse Anti-PPIA Recombinant Antibody (clone 30F8) | ELISA, WB | Mouse IgG |
| MOB-0119CT | Recombinant Mouse anti-Human PPIA Monoclonal antibody (EML2216) | ELISA, ICC, IF, WB | |
| MRO-0419-CN | Recombinant Rabbit Anti-PPIA Monoclonal Antibody (JM107-03) | WB, IHC | Rabbit IgG |
| MOB-286YJL | Rabbit Anti-PPIA Recombinant Antibody (clone CBP271) | ELISA | Rabbit IgG |
| VS13-YC922 | Rabbit Anti-PPIA Recombinant Antibody (VS13-YC922) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2804 | Hi-Affi™ Recombinant Rabbit Anti-PPIA Monoclonal Antibody (DS2804AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1153 | AbPlus™ Anti-PPIA Magnetic Beads (VS-0724-YC1153) | IP, Protein Purification | |
| VS-0724-YC1230 | AbPlus™ Anti-Ppia Magnetic Beads (VS-0724-YC1230) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1708 | Anti-PPIA Immunohistochemistry Kit | IHC |
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