Rabbit Anti-PPIF Recombinant Antibody (clone R02-6I9)
CAT#: VS3-FY1691
The R02-6I9 recombinant monoclonal antibody binds to human PPIF. It cross-reacts with mouse, rat. The antibody is recommended for detection of PPIF protein by WB, IHC-P. The specificity of the cyclophilin F antibody was confirmed by western blot analysis of cyclophilin F in K562, 3T3, Hela lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-PPIF Antibody (VS3-FY1691) in WB.
The Cyclophilin F expression in K562, 3T3, Hela lysates were detected using the anti-Cyclophilin F (PPIF) by western blot assay.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/77416/1717_C3_13_cr57f4fa75c837b_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108179-PPIF/subcellular
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Specifications
- Immunogen
- Synthetic peptide of human cyclophilin F
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PPIF
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-6I9
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 22 kDa, while the observed molecular weight stands at 18 kDa.
- Related Disease
- Ullrich Congenital Muscular Dystrophy 1 and Bethlem Myopathy 1
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- CYP3; CypD; CyP-M; Cyp-D
- Gene ID
- 10105
- UniProt ID
- P30405
- Sequence Similarities
- Belongs to the cyclophilin-type PPIase family.
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Acetylated at Lys-167; deacetylated at Lys-167 by SIRT3.
- Protein Refseq
- XP_005269436.1; XP_054220447.1; NP_005720.1
- Function
- It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PPIF"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0121CT | Recombinant Mouse anti-Human PPIF Monoclonal antibody (BU2G6) | ELISA, IHC-P, WB | |
| MRO-0421-CN | Recombinant Rabbit Anti-PPIF Monoclonal Antibody (JM71-39) | WB, IHC, IP | Rabbit IgG |
| VS3-WK606 | Rabbit Anti-PPIF Recombinant Antibody (clone JM71-39) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0434MZ | Chicken Anti-PPIF Polyclonal IgY | Indirect ELISA, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2808 | Hi-Affi™ Recombinant Rabbit Anti-PPIF Monoclonal Antibody (DS2808AB) | WB, IHC-P, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1711 | Anti-PPIF Immunohistochemistry Kit | IHC | |
| VS-0525-XY5601 | Anti-Mouse PPIF Immunohistochemistry Kit | IHC | |
| VS-0525-XY5600 | Anti-Human PPIF Immunohistochemistry Kit | IHC | |
| VS-0525-XY5602 | Anti-Rat PPIF Immunohistochemistry Kit | IHC |
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