Rabbit Anti-PPIF Recombinant Antibody (clone R02-6I9) (CAT#: VS3-FY1691)
The R02-6I9 recombinant monoclonal antibody binds to human PPIF. It cross-reacts with mouse, rat. The antibody is recommended for detection of PPIF protein by WB, IHC-P. The specificity of the cyclophilin F antibody was confirmed by western blot analysis of cyclophilin F in K562, 3T3, Hela lysates.
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WB
Figure 1 Anti-PPIF Antibody (VS3-FY1691) in WB.
The Cyclophilin F expression in K562, 3T3, Hela lysates were detected using the anti-Cyclophilin F (PPIF) by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human cyclophilin F
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PPIF
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-6I9
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 22 kDa, while the observed molecular weight stands at 18 kDa.
- Related Disease
- Ullrich Congenital Muscular Dystrophy 1 and Bethlem Myopathy 1
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- CYP3; CypD; CyP-M; Cyp-D
- Gene ID
- 10105
- UniProt ID
- P30405
- Sequence Similarities
- Belongs to the cyclophilin-type PPIase family.
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Acetylated at Lys-167; deacetylated at Lys-167 by SIRT3.
- Protein Refseq
- XP_005269436.1; XP_054220447.1; NP_005720.1
- Function
- It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibodies
Chicken IgY Antibodies
Rabbit Monoclonal Antibodies
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0121CT | Recombinant Mouse anti-Human PPIF Monoclonal antibody (BU2G6) | ELISA, IHC-P, WB | |
| MRO-0421-CN | Recombinant Rabbit Anti-PPIF Monoclonal Antibody (JM71-39) | WB, IHC, IP | Rabbit IgG |
| VS3-WK606 | Rabbit Anti-PPIF Recombinant Antibody (clone JM71-39) | WB, ELISA | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
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