Rabbit Anti-PPIF Recombinant Antibody (clone R02-6I9) (CAT#: VS3-FY1691)
The R02-6I9 recombinant monoclonal antibody binds to human PPIF. It cross-reacts with mouse, rat. The antibody is recommended for detection of PPIF protein by WB, IHC-P. The specificity of the cyclophilin F antibody was confirmed by western blot analysis of cyclophilin F in K562, 3T3, Hela lysates.
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WB
Figure 1 Anti-PPIF Antibody (VS3-FY1691) in WB.
The Cyclophilin F expression in K562, 3T3, Hela lysates were detected using the anti-Cyclophilin F (PPIF) by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human cyclophilin F
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PPIF
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-6I9
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 22 kDa, while the observed molecular weight stands at 18 kDa.
- Related Disease
- Ullrich Congenital Muscular Dystrophy 1 and Bethlem Myopathy 1
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- CYP3; CypD; CyP-M; Cyp-D
- Gene ID
- 10105
- UniProt ID
- P30405
- Sequence Similarities
- Belongs to the cyclophilin-type PPIase family.
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Acetylated at Lys-167; deacetylated at Lys-167 by SIRT3.
- Protein Refseq
- XP_005269436.1; XP_054220447.1; NP_005720.1
- Function
- It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0121CT | Recombinant Mouse anti-Human PPIF Monoclonal antibody (BU2G6) | ELISA, IHC-P, WB | |
| MRO-0421-CN | Recombinant Rabbit Anti-PPIF Monoclonal Antibody (JM71-39) | WB, IHC, IP | Rabbit IgG |
| VS3-WK606 | Rabbit Anti-PPIF Recombinant Antibody (clone JM71-39) | WB, ELISA | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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