Mouse Anti-PPP4C Recombinant Antibody (VS3-CJ75) (CAT#: VS3-CJ75)

• Immunogen
• Recombinant full length of human PPP4C

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human PPP4C

• Species Reactivity
• Human

• Applications
• WB, ELISA

• Conjugate
• Unconjugated

• Purification
• Protein G affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Concentration
• 1 mg/mL (lot specific)

• Buffer
• 50% Glycerol, 1% BSA, PBS, pH7.4.

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).

• Alternative Names
• PP-X; PP4; PP4C; PPH3; PPP4; PPX

• Gene ID
• 5531

• UniProt ID
• P60510

• Sequence Similarities
• Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.

• Cellular Localization
• Nucleus, Cytoplasm, Cytoskeleton

• Post Translation Modifications
• Methylation at the C-terminal Leu-307 is critical for interactions with regulatory subunits and functions in DNA repair.

• Protein Refseq
• NP_001290432.1; NP_001290433.1; NP_001290435.1

• Function
• Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on Ser-140 (gamma-H2AX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity).
  In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS