What format is this antibody supplied in?

This product is a Fab Fragment of a recombinant Human antibody. It lacks the Fc region, which can be advantageous for reducing non-specific binding in assays like ELISA, WB, and RIA.

What is the specificity of this antibody?

It specifically recognizes PRAME (Preferentially Expressed Antigen in Melanoma). This antigen is typically expressed in melanoma and testis, making this antibody a useful tool for cancer research.

Human Anti-PRAME Recombinant Antibody; Fab Fragment (HPAB-1710LY-F(E))

CAT#: HPAB-1710LY-F(E)

This product is a recombinant Human antibody Fab Fragment that recognizes PRAME. The antibody was purified by affinity chromatography. It can be used for ELISA, WB, RIA and other applications.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line K-562

Immunofluorescent staining of human cell line K-562 shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68562/1854_A5_4_selected.jpg

Normal Tissue Figure 2 Colon

Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45153/160303_A_7_3.jpg

Normal Tissue Figure 3 Testis

Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45153/160303_A_5_6.jpg

RNA Expression Figure 4 RNA cell line category: Cell line enriched (K-562)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185686-PRAME/subcellular

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Specifications

Host Species
Human

Type
Humanized Fab

Specificity
Human PRAME

Species Reactivity
Human

Applications
ELISA, WB, RIA

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
MAPE; OIP4; CT130; OIP-4

Gene ID
23532

UniProt ID
P78395

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Breast cancer biomarkers at key points during disease progression

Useful Fab Fragment

We specifically needed a Fab fragment for our PRAME research to avoid Fc-mediated non-specific binding. This product worked perfectly in our Western Blot analysis. It recognized the antigen in our melanoma cell lysates clearly. The affinity purification really ensures a high-quality reagent for research use.

Great For Antigen Detection

This antibody fragment was a key component in our RIA setup. It showed excellent specificity for the PRAME antigen, which is not expressed in most normal tissues. The data we generated was clean and reproducible. It is a valuable asset for anyone studying cancer-testis antigens.

Q&As

What format is this antibody supplied in?

A: This product is a Fab Fragment of a recombinant Human antibody. It lacks the Fc region, which can be advantageous for reducing non-specific binding in assays like ELISA, WB, and RIA.
What is the specificity of this antibody?

A: It specifically recognizes PRAME (Preferentially Expressed Antigen in Melanoma). This antigen is typically expressed in melanoma and testis, making this antibody a useful tool for cancer research.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-1710LY-F(E).pdf

MSDS

MSDS - HPAB-1710LY-F(E).pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

See other products for "PRAME"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
MHC-LC181	PE-A*02:01/Human PRAME (ALYVDSLFFL) MHC Tetramer	FCM

CAT	Product Name	Application	Type
MHC-LC182	APC-A*02:01/Human PRAME (ALYVDSLFFL) MHC Tetramer	FCM

CAT	Product Name	Application	Type
MHC-LC183	PE-A*02:01/Human PRAME (VLDGLDVLL) MHC Tetramer	FCM

CAT	Product Name	Application	Type
MHC-LC184	APC-A*02:01/Human PRAME (VLDGLDVLL) MHC Tetramer	FCM

CAT	Product Name	Application	Type
MHC-LC185	PE-A*02:01/Human PRAME (SLLQHLIGL) MHC Tetramer	FCM

CAT	Product Name	Application	Type
HPAB-1701LY-S(P)	Human Anti-PRAME Recombinant Antibody; scFv Fragment (HPAB-1701LY-S(P))	ELISA, WB, RIA	Humanized scfv

CAT	Product Name	Application	Type
HPAB-1702LY-S(P)	Human Anti-PRAME Recombinant Antibody; scFv Fragment (HPAB-1702LY-S(P))	ELISA, WB, RIA	Humanized scfv

CAT	Product Name	Application	Type
HPAB-1703LY-S(P)	Human Anti-PRAME Recombinant Antibody; scFv Fragment (HPAB-1703LY-S(P))	ELISA, WB, RIA	Humanized scfv

CAT	Product Name	Application	Type
HPAB-1704LY-S(P)	Human Anti-PRAME Recombinant Antibody; scFv Fragment (HPAB-1704LY-S(P))	ELISA, WB, RIA	Humanized scfv

CAT	Product Name	Application	Type
HPAB-1705LY-S(P)	Human Anti-PRAME Recombinant Antibody; scFv Fragment (HPAB-1705LY-S(P))	ELISA, WB, RIA	Humanized scfv

CAT	Product Name	Application	Type
HPAB-1701LY	Human Anti-PRAME Recombinant Antibody (HPAB-1701LY)	ELISA, WB, RIA	Humanized IgG

CAT	Product Name	Application	Type
HPAB-1702LY	Human Anti-PRAME Recombinant Antibody (HPAB-1702LY)	ELISA, WB, RIA	Humanized IgG

CAT	Product Name	Application	Type
HPAB-1703LY	Human Anti-PRAME Recombinant Antibody (HPAB-1703LY)	ELISA, WB, RIA	Humanized IgG

CAT	Product Name	Application	Type
HPAB-1704LY	Human Anti-PRAME Recombinant Antibody (HPAB-1704LY)	ELISA, WB, RIA	Humanized IgG

CAT	Product Name	Application	Type
HPAB-1705LY	Human Anti-PRAME Recombinant Antibody (HPAB-1705LY)	ELISA, WB, RIA	Humanized IgG

CAT	Product Name	Application	Type
HPAB-1701LY-F(E)	Human Anti-PRAME Recombinant Antibody; Fab Fragment (HPAB-1701LY-F(E))	ELISA, WB, RIA	Humanized Fab

CAT	Product Name	Application	Type
HPAB-1702LY-F(E)	Human Anti-PRAME Recombinant Antibody; Fab Fragment (HPAB-1702LY-F(E))	ELISA, WB, RIA	Humanized Fab

CAT	Product Name	Application	Type
HPAB-1703LY-F(E)	Human Anti-PRAME Recombinant Antibody; Fab Fragment (HPAB-1703LY-F(E))	ELISA, WB, RIA	Humanized Fab

CAT	Product Name	Application	Type
HPAB-1704LY-F(E)	Human Anti-PRAME Recombinant Antibody; Fab Fragment (HPAB-1704LY-F(E))	ELISA, WB, RIA	Humanized Fab

CAT	Product Name	Application	Type
HPAB-1705LY-F(E)	Human Anti-PRAME Recombinant Antibody; Fab Fragment (HPAB-1705LY-F(E))	ELISA, WB, RIA	Humanized Fab

CAT	Product Name	Application	Type
VS-0525-XY5648	Anti-PRAME Immunohistochemistry Kit	IHC

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