Rabbit Anti-PRKAA2 (pS345) Recombinant Antibody (clone R04-5T2)
CAT#: VS3-WK2151
This product is a rabbit recombinant antibody that is specific to phosphorylated PRKAA2. The target of this antibody is phospho-PRKAA2 (Ser345). The clone R04-5T2 can be used in WB analysis at a dilution of 1/500-1/1000.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear speckles & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44540/1581_D4_3_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44540/125371_B_6_6_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44540/125371_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44540/125371_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44540/125371_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Pachytene spermatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44540/125371_A_6_6.jpg
Normal Tissue
Figure 7 Skeletal muscle
Myocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44540/125371_B_2_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (RPTEC TERT1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162409-PRKAA2/subcellular
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Ser345 of human, mouse and rat PRKAA2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-5T2
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "PRKAA2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2845 | Hi-Affi™ Recombinant Rabbit Anti-PRKAA2 Monoclonal Antibody (DS2845AB) | ELISA, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0519U | Rabbit Anti-Phospho-PRKAA2 (Thr172) Recombinant Antibody (clone 4F4) | ELISA, WB, IF | Rabbit IgG |
| ZG-0520U | Rabbit Anti-Phospho-PRKAA2 (S491) Recombinant Antibody (clone 3H10) | ELISA, WB | Rabbit IgG |
| VS3-QX915 | Mouse Anti-PRKAA2 Recombinant Antibody (clone 4B11-G3-G10) | WB, ICC | Mouse IgG2a, κ |
| VS3-WK170 | Mouse Anti-PRKAA2 Recombinant Antibody (clone H5-E11) | WB, ELISA | Mouse IgG1 |
| VS3-CJ1038 | Rabbit Anti-PRKAA2 (phospho S345) Recombinant Antibody (VS3-CJ1038) | WB, ICC, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1742 | Anti-PRKAA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5673 | Anti-Human PRKAA2 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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