Rabbit Anti-PRKAG1 Recombinant Antibody (VS13-YC930) (CAT#: VS13-YC930)

• Immunogen
• Recombinant human AMPK gamma 1.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human PRKAG1

• Species Reactivity
• Human

• Applications
• WB, IF, IP

• Conjugate
• NONE

• Related Disease
• Cardiovascular Disease

• Clonality
• Monoclonal

• Purification
• Affinity Chromatography purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 50 mM Tris-Glycine (pH 7.4) containing 150 mM NaCl, 40% Glycerol and 0.05% BSA.

• Preservative
• 0.01% Sodium azide

• Storage
• Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipping with ice pack.

• Application Notes
• Western blot (WB): 1/500-1/1000
  Immunofluorescence (IF): 1/50-1/200
  Immunoprecipitation (IP): 1/20

• Alternative Names
• AMPKG

• Gene ID
• 5571

• UniProt ID
• P54619

• Long Name
• protein kinase AMP-activated non-catalytic subunit gamma 1

• Sequence Similarities
• Belongs to the 5'-AMP-activated protein kinase gamma subunit family.

• Cellular Localization
• Cytosol; Nucleoplasm; Nucleus

• Protein Refseq
• NP_001193638.1; NP_001193639.1; NP_002724.1

• Function
• AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.

Secondary Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

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