CytoStream™ Rabbit Anti-PRKCB Recombinant Antibody (VS13-YC936)
CAT#: VS13-YC936
The CytoStream™ Rabbit Anti-PRKCB Recombinant Antibody is specific for human and mouse PRKCB. PRKCB is another PKC family member, involved in varied cellular functions such as B cell activation, with specificity in signaling roles. The anti-PRKCB antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Specifications
- Immunogen
- Recombinant human PKC beta 1, aa 608-657.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse PRKCB
- Species Reactivity
- Human, Mouse
- Applications
- WB, IHC-P, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- PRKCB2, PKCB, PRKCB1
- Gene ID
- 5579
- UniProt ID
- P05771
- Long Name
- Protein Kinase C Beta
- Sequence Similarities
- Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
- Cellular Localization
- Nucleus; Cytoplasm; Cell membrane
- Protein Refseq
- NP_002729.2; NP_997700.1
- Function
- Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity (PubMed:11598012).
Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A (PubMed:20228790).
In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. Participates in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Phosphorylates SLC2A1/GLUT1, promoting glucose uptake by SLC2A1/GLUT1 (PubMed:25982116).
Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity).
Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription (PubMed:19176525).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PRKCB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2854 | Hi-Affi™ Recombinant Rabbit Anti-PRKCB Monoclonal Antibody (DS2854AB) | IHC-P, WB, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1236-CN | Recombinant Rabbit Anti-PRKCB Monoclonal Antibody (JM43-26) | WB, IHC, FC | Rabbit IgG |
| MRO-1237-CN | Recombinant Rabbit Anti-PRKCB Monoclonal Antibody (CBACN-451) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0413U | Rabbit Anti-PRKCB Recombinant Antibody (clone N20-N), FITC | FC | Rabbit IgG |
| ZG-0414U | Rabbit Anti-PRKCB Recombinant Antibody (clone Q19-V), FITC | FC | Rabbit IgG |
| VS3-CJ420 | Rabbit Anti-PRKCB Recombinant Antibody (VS3-CJ420) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY217 | AbPlus™ Anti-PRKCB Magnetic Beads (CBACN-451) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC937 | CytoStream™ Rabbit Anti-PRKCB Recombinant Antibody (VS13-YC937) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1750 | Anti-PRKCB Immunohistochemistry Kit | IHC | |
| VS-0525-XY5691 | Anti-Mouse PRKCB Immunohistochemistry Kit | IHC | |
| VS-0525-XY5690 | Anti-Human PRKCB Immunohistochemistry Kit | IHC |
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