Rabbit Anti-PRKCQ Recombinant Antibody (clone R05-5I4)
CAT#: VS3-FY2064
The R05-5I4 recombinant monoclonal antibody has the ability to specifically bind to human PRKCQ. It cross-reacts with rat. The binding specificity of the PKC theta antibody was determined using western blot analysis of PKC theta/PRKCQ in U2OS, C6 lysates.
Tested Data
WB
Figure 1 Anti-PRKCQ Antibody (VS3-FY2064) in WB.
The PKC theta/PRKCQ expression in U2OS, C6 lysates were detected using the anti-PKC theta by western blot assay.
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Specifications
- Immunogen
- Synthetic peptides of human PKC theta/PRKCQ
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat PRKCQ
- Species Reactivity
- Human, Rat
- Clone
- R05-5I4
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 82 kDa, while the observed molecular weight stands at 79 kDa.
- Related Disease
- Inflammatory Bowel Disease 1 and Gastrointestinal Stromal Tumor
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- PRKCT; nPKC-theta
- Gene ID
- 5588
- UniProt ID
- Q04759
- Sequence Similarities
- Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane
- Post Translation Modifications
- Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PRKCQ upon antigen receptor ligation.
Following TCR stimulation, phosphorylated at Tyr-90 and Ser-685.
(Q04759-KPCT_HUMAN)
Ubiquitination at Lys77, Lys110, Lys286, Lys325, Lys384, and Lys527 (NX_Q04759 [NX_Q04759-1])
Modification sites at PhosphoSitePlus (Q04759)
Glycosylation from GlyGen (Q04759) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_005252553.1; XP_005252554.1; XP_054222269.1; XP_054222270.1; NP_001229342.1
- Function
- Plays a critical role in TCR/CD28-induced NFATC1 and NFATC2 transactivation by participating in the regulation of reduced inositol 1,4,5-trisphosphate generation and intracellular calcium mobilization. After costimulation of T-cells through CD28 can phosphorylate CBLB and is required for the ubiquitination and subsequent degradation of CBLB, which is a prerequisite for the activation of TCR. During T-cells differentiation, plays an important role in the development of T-helper 2 (Th2) cells following immune and inflammatory responses, and, in the development of inflammatory autoimmune diseases, is necessary for the activation of IL17-producing Th17 cells. May play a minor role in Th1 response.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PRKCQ"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3032z | Mouse Anti-PRKCQ Recombinant Antibody (clone 16G9) | ELISA, WB, FuncS | Mouse IgG1 |
| MOB-2308CT | Recombinant Mouse anti-Human PRKCQ Monoclonal antibody (FQS4905) | WB, ELISA | |
| ZG-0004U | Rabbit Anti-PRKCQ Recombinant Antibody (clone 1B2) | ELISA, WB | Rabbit IgG |
| VS13-YC941 | Rabbit Anti-PRKCQ Recombinant Antibody (VS13-YC941) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0438MZ | Chicken Anti-PRKCQ Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2858 | Hi-Affi™ Recombinant Rabbit Anti-PRKCQ Monoclonal Antibody (DS2858AB) | WB | IgG |
| MOR-4702 | Hi-Affi™ Recombinant Rabbit Anti-PRKCQ Monoclonal Antibody (TH216DS) | WB, IHC-P, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1754 | Anti-PRKCQ Immunohistochemistry Kit | IHC |
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