Human Anti-PRNP Recombinant Antibody (clone D18) (CAT#: FAMAB-0294-CN)

Figure 1 Antibodies recognizing the C-terminal domain of PrPC induce dendritic degeneration in hippocampal neurons.

Top, representative images showing dendrite morphology of cultured hippocampal neurons from Tga20 mice (which over-express WT PrPC), WT mice, or Prnp−/− mice after treatment for 48 hr with D18 (16.7 nM). The cells were stained with an antibody to MAP2 to visualize dendrites. Boxed areas are enlarged below each image. Scale bar = 10 µm. Bottom, quantitation of dendritic degeneration, expressed as the length of beaded dendrite segments as a percentage of total dendrite length, from 10 images in three independent cultures for each experimental condition. Data represent mean ± S.E.M. **p<0.01; ***p<0.005.

Wu, B., McDonald, A. J., Markham, K., Rich, C. B., McHugh, K. P., Tatzelt, J., ... & Harris, D. A. (2017). The N-terminus of the prion protein is a toxic effector regulated by the C-terminus. Elife, 6, e23473.