AbPlus™ Anti-PSMA1 Magnetic Beads (JB89-30)
CAT#: VS-0424-XY218
The AbPlus Anti-PSMA1 Magnetic Beads (JB89-30) is an innovative affinity resin which is bound with anti-PSMA1 specific antibody (JB89-30). The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of PSMA1 protein under native and denaturing conditions.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37646/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows distinct cytoplasmic and nuclear positivity in cells in seminiferus ducts and Leydig cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37646/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37646/78642_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37646/78642_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37646/78642_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37646/78642_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37646/78642_A_5_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37646/78642_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000129084-PSMA1/subcellular
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magrose bead (> 50 μmol/mL gel)
- Bead Ligand
- Anti-PSMA1 specific antibody (JB89-30)
- Target
- PSMA1
- Immunogen
- Recombinant protein within human PSMA1 (aa 1-200)
- Target Species
- Human, Mouse, Rat
- Antibody Clone
- JB89-30
- Bead Capacity
- 20-30 mg/mL binding antibody
- Bead size
- 10-37 μm
- Stability
- pH 2-14
- Format
- 20% Suspension
- Buffer
- PBS, pH 7.4, with 1% BSA
- Preservative
- 0.03% Proclin 300
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
- Alternative Names
- NU; HC2; PROS30; HEL-S-275
- Gene ID
- 5682
- UniProt ID
- P25786
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Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "PSMA1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3070z | Mouse Anti-PSMA1 Recombinant Antibody (clone 36H6) | WB, ELISA, ICC, IF, IHC | Mouse IgG2b |
| MOB-1707CT | Recombinant Mouse anti-Human PSMA1 Monoclonal antibody (NDQ30) | ICC, IF, IP, WB | |
| MRO-1277-CN | Recombinant Rabbit Anti-PSMA1 Monoclonal Antibody (JB89-30) | WB, IHC, FC, IP | Rabbit IgG |
| MRO-1288-CN | Recombinant Rabbit Anti-PSMA1 Monoclonal Antibody (JU66-24) | WB, IF, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0443MZ | Chicken Anti-PSMA1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2887 | Hi-Affi™ Recombinant Rabbit Anti-PSMA1 Monoclonal Antibody (DS2887AB) | WB, IHC, ICC, IP, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1398 | AbPlus™ Anti-PSMA1 Magnetic Beads (VS-0724-YC1398) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1775 | Anti-PSMA1 Immunohistochemistry Kit | IHC |
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