Rabbit Anti-PSMA2 Recombinant Antibody (clone R09-4H8) (CAT#: VS3-FY1854)

The R09-4H8 recombinant monoclonal antibody exhibits specific binding to human PSMA2. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. The Proteasome alpha 2 antibody has been teted positive by western blot analysis of proteasome 20S alpha 2 in mouse spleen, 3T3 lysates and immunohistochemistry analysis of paraffin-embedded human tonsil.


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WB

Figure 1 Anti-PSMA2 Antibody (VS3-FY1854) in WB.

Figure 1 Anti-PSMA2 Antibody (VS3-FY1854) in WB.

The Proteasome 20S alpha 2 expression in mouse spleen, 3T3 lysates were detected using the anti-Proteasome 20S alpha 2 by western blot assay.

IHC

Figure 2 Anti-PSMA2 Antibody (VS3-FY1854) in IHC.

Figure 2 Anti-PSMA2 Antibody (VS3-FY1854) in IHC.

Immunohistochemistry staining of paraffin-embedded human tonsil using the anti-Proteasome 20S alpha 2 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.


Specifications

  • Immunogen
  • Synthetic peptide of human proteasome 20S α 2
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat PSMA2
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R09-4H8
  • Applications
  • Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
  • MW
  • The calculated weight of the target protein is approximately 26 kDa, while the observed molecular weight stands at 26 kDa.
  • Related Disease
  • Cystic Fibrosis and Charcot-Marie-Tooth Disease, Axonal, Type 2E

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/50-1/100
    Immunofluorescence: 1/50-1/200

Target

  • Alternative Names
  • MU; HC3; PSC2; PMSA2
  • Sequence Similarities
  • Belongs to the peptidase T1A family.
  • Cellular Localization
  • Cytoplasm, Nucleus, Proteasome
  • Post Translation Modifications
  • Phosphorylated on tyrosine residues; which may be important for nuclear import. (P25787-PSA2_HUMAN)
    Ubiquitination at Lys39, Lys53, Lys70, Lys92, Lys165, and Lys171 (NX_P25787 [NX_P25787-1])
    Modification sites at PhosphoSitePlus (P25787)
    Glycosylation from GlyGen (P25787) 1 site, 1 O-linked glycan (1 site)
  • Function
  • The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation.

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "PSMA2"

Recombinant Antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2888 Hi-Affi™ Recombinant Rabbit Anti-PSMA2 Monoclonal Antibody (DS2888AB) WB, IHC, ICC, FC IgG

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC4420 PE-B*58:01/Human PSMA2 (DPSGAYFAW) MHC Tetramer FCM

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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