Mouse Anti-PSMC3 Recombinant Antibody (clone 1G10B7) (CAT#: VS7-0425-WR705)

• Immunogen
• Recombinant human PSMC3 (AA: 53-152) fragment expressed in E. Coli.

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human PSMC3

• Species Reactivity
• Human, Monkey, Rat

• Clone
• 1G10B7

• Molecule Class
• 26S Proteasome

• Applications
• WB, IHC, ICC, FC

• Conjugate
• Unconjugated

• Related Disease
• Deafness, cataract, impaired intellectual development, and polyneuropathy (DCIDP)

• Clonality
• Monoclonal

• Purity
• >95% as determined by SDS-PAGE and SEC-HPLC analysis

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS

• Preservative
• 0.05% sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• Western Blot: 1:500 - 1:2000
  Immunohistochemistry-Paraffin: 1:200 - 1:1000
  Immunocytochemistry: 1:200 - 1:1000
  Flow Cytometry: 1:200 - 1:400
  Enzyme-linked Immunosorbent Assay: 1:10000

• Alternative Names
• PSMC3; Proteasome 26S Subunit ATPase 3; Proteasome Subunit P50; TBP-1; Human Immunodeficiency Virus Tat Transactivator Binding Protein-1; Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 3; Tbp1

• Gene ID
• 5702

• UniProt ID
• P17980

• Long Name
• Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 3

• Cellular Localization
• Cytoplasm; Nucleus; Proteasome

• Post Translation Modifications
• Acetylation; Phosphoprotein; Ubl conjugation

• Protein Refseq
• NP_002795.2

• Function
• Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC3 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Secondary Antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Reconstitution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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