AbPlus™ Anti-PTBP1 Magnetic Beads (VS-0724-YC963)
CAT#: VS-0724-YC963
The AbPlus Anti-PTBP1 Magnetic Beads (VS-0724-YC963) is an innovative affinity resin which is bound with anti-PTBP1 specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of PTBP1 protein under native and denaturing conditions.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13507/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13507/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13507/30256_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13507/30256_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13507/30256_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Peritubular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13507/30256_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13507/30256_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000011304-PTBP1/subcellular
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-PTBP1 specific antibody
- Target
- PTBP1
- Immunogen
- Recombinant human PSME3/PA28-gamma, aa 1-254.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring.
- Alternative Names
- Ki; PA28G; HEL-S-283; PA28gamma; REG-GAMMA; PA28-gamma
- Gene ID
- 10197
- UniProt ID
- P61289
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "PTBP1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1658CT | Recombinant Mouse anti-Human PTBP1 Monoclonal antibody (EML2203) | IHC-P, WB | |
| MRO-1290-CN | Recombinant Mouse Anti-PTBP1 Monoclonal Antibody (9A1) | WB, IHC, IF, FC | Mouse IgG2a |
| MRO-2143-CN | Rabbit Anti-PTBP1 Polyclonal Antibody (MRO-2143-CN) | WB, IF, IHC | Rabbit IgG |
| ZG-0526U | Rabbit Anti-PTBP1 Recombinant Antibody (clone 8A1) | ELISA, WB, IHC | Rabbit IgG |
| VS3-FY1210 | Recombinant Rabbit Anti-PTBP1 Antibody (clone R01-1G2) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2911 | Hi-Affi™ Recombinant Rabbit Anti-PTBP1 Monoclonal Antibody (DS2911AB) | WB, IHC, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2086 | PE-B*07:02/Human PTBP1 (SPNQARAQAAL) MHC Tetramer | FCM | |
| MHC-LC2087 | APC-B*07:02/Human PTBP1 (SPNQARAQAAL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1815 | Anti-PTBP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5812 | Anti-Mouse PTBP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5811 | Anti-Human PTBP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5813 | Anti-Rat PTBP1 Immunohistochemistry Kit | IHC |
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