Mouse Anti-PTK2 Recombinant Antibody (ZG-0331J)
CAT#: ZG-0331J
This product is a mouse antibody that recognizes PTK2.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-PTK2 Recombinant Antibody (ZG-0331J) in WB
Western Blot analysis using FAK Monoclonal Antibody against A549 (1) and NIH/3T3 (2) cell lysate.
IHC
Figure 2 Mouse Anti-PTK2 Recombinant Antibody (ZG-0331J) in IHC
Immunohistochemistry analysis of paraffin-embedded cervices tumour with DAB staining using FAK Monoclonal Antibody.
IF
Figure 3 Mouse Anti-PTK2 Recombinant Antibody (ZG-0331J) in IF
Immunofluorescence analysis of B16 cells using FAK Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_6_5_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_6_6.jpg
Normal Tissue
Figure 8 Breast
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Myoepithelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_B_1_4.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169398-PTK2/subcellular
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Specifications
- Immunogen
- Purified recombinant fragment of human FAK expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PTK2
- Species Reactivity
- Human, Mouse
- Applications
- WB, IHC, IF, ELISA
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunohistochemistry, Immunofluorescence, Enzyme-linked Immunosorbent Assay.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| MOR-2924 | Hi-Affi™ Recombinant Rabbit Anti-PTK2 Monoclonal Antibody (DS2924AB) | ICC, WB | IgG |
| MOR-4573 | Hi-Affi™ Recombinant Rabbit Anti-PTK2 Monoclonal Antibody (TH85DS) | WB, IF, ICC, FC | IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2719 | PE-A*02:01/Human PTK2 (SLDLASLIL) MHC Tetramer | FCM | |
| MHC-LC2720 | PE-A*02:07/Human PTK2 (SLDLASLIL) MHC Tetramer | FCM | |
| MHC-LC4514 | PE-Mamu-A1*001:01/Human PTK2 (LTAAHALAV) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0100U | Rabbit Anti-PTK2 Recombinant Antibody (clone 3B2) | ELISA, IHC | Rabbit IgG |
| VS3-QX933 | Mouse Anti-PTK2 Recombinant Antibody (clone 3D8-H11-E12) | WB, IF, IP | Mouse IgG1 |
| VS3-XY1315 | Mouse Anti-PTK2 Recombinant Antibody (clone 4G1) | WB, IP, IF, IHC | Mouse IgG1 |
| VS3-XY1316 | Mouse Anti-PTK2 Recombinant Antibody (clone 4A9D6) | ELISA, WB | Mouse IgG1 |
| VS3-XY1317 | Mouse Anti-PTK2 Recombinant Antibody (clone 4B5D2) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY219 | AbPlus™ Anti-PTK2 Magnetic Beads (CBACN-222) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC956 | CytoStream™ Rabbit Anti-PTK2 Recombinant Antibody (VS13-YC956) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5845 | Anti-PTK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5846 | Anti-Mouse PTK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5847 | Anti-Rat PTK2 Immunohistochemistry Kit | IHC |
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