AbPlus™ Anti-PTPRC Magnetic Beads (VS-0724-YC1181)

Name: AbPlus™ Anti-PTPRC Magnetic Beads (VS-0724-YC1181)
SKU: VS-0724-YC1181
Rating: 5 (1 reviews)

CAT#: VS-0724-YC1181

The AbPlus Anti-PTPRC Magnetic Beads (VS-0724-YC1181) is an innovative affinity resin which is bound with anti-PTPRC specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of PTPRC protein under native and denaturing conditions.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/440/if_selected.jpg

Normal Tissue Figure 2 Appendix

Lymphoid tissue
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/440/155132_A_3_2.jpg

Normal Tissue Figure 3 Lymph node

Germinal center cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/440/155132_A_8_8.jpg

RNA Expression Figure 4 RNA cell line category: Cell line enhanced (Daudi, HEL, HL-60, JURKAT, MOLT-4, THP-1, U-266/70, U-266/84, U-698, U-937)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000081237-PTPRC

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Specifications

Applications
Immunoprecipitation, Protein Purification

Matrix
Magnetic bead

Bead Ligand
Anti-PTPRC specific antibody

Target
PTPRC

Immunogen
Recombinant human CD45 fragment derived from E. coli.

Target Species
Human

Bead Capacity
40 mg/mL

Bead size
25 μm

Format
Suspension

Concentration
2 mg/mL

Buffer
PBS, pH 7.4

Preservative
0.1% Sodium azide

Storage
Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.

Applications

Application Notes
The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.

Target

Introduction
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling.

Alternative Names
LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180; IMD105

Gene ID
5788

UniProt ID
P08575

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Downloadable Resources

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Datasheet

Datasheet - VS-0724-YC1181.pdf

MSDS

MSDS - VS-0724-YC1181.pdf

COA

Certificate of Analysis Lookup

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