Mouse Anti-PTPRC Recombinant Antibody (clone 17H9D4) (CAT#: ZG-0253U)

Figure 1 Mouse Anti-PTPRC Antibody (ZG-0253U) in WB

Positive WB detected in: Jurkat whole cell lysate, Raji whole cell lysate, THP-1 whole cell lysate All lanes CD45 antibody at 1: 2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 148, 132, 143, 141, 139, 136 KDa
Observed band size: 180-250 KDa
Exposure time: 15min

Figure 2 Mouse Anti-PTPRC Antibody (ZG-0253U) in IHC

IHC image of ZG-0253U diluted at 1:500 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.

Figure 3 Mouse Anti-PTPRC Antibody (ZG-0253U) in IF

Immunofluorescence staining of Raji cells with ZG-0253U at 1:250, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Mouse IgG (H+L).

Figure 4 Mouse Anti-PTPRC Antibody (ZG-0253U) in FC

Overlay histogram showing Jurkat cells stained with ZG-0253U (red line) at 1:500. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.