Rabbit Anti-PUS1 Recombinant Antibody (clone R02-0A2) (CAT#: VS3-FY1862)
Clone R02-0A2 is a rabbit recombinant monoclonal antibody that with high affinity for human PUS1. It cross-reacts with hamster, rat. It is suitable for use in a variety of assays, including WB, ICC/IF, IP.
Recommended dilution is western blot (dilution 1/500-1/1000), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20).
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WB
Figure 1 Anti-PUS1 Antibody (VS3-FY1862) in WB.
The PUS1 expression in Jurkat, C6, CHO-K1, Hela lysates were detected using the anti-PUS1 by western blot assay.
Specifications
- Immunogen
- Recombinant human PUS1 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Hamster, Rat PUS1
- Species Reactivity
- Human, Hamster, Rat
- Clone
- R02-0A2
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 47 kDa, while the observed molecular weight stands at 44,47 kDa.
- Related Disease
- Myopathy, Lactic Acidosis, And Sideroblastic Anemia 1 and Myopathy, Lactic Acidosis, And Sideroblastic Anemia
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- MLASA1
- Gene ID
- 80324
- UniProt ID
- Q9Y606
- Sequence Similarities
- Belongs to the tRNA pseudouridine synthase TruA family.
- Cellular Localization
- Cytoplasm, Mitochondrion, Nucleus
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q9Y606)
Modification sites at neXtProt (NX_Q9Y606)
Glycosylation from GlyGen (Q9Y606) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001002019.1; NP_001002020.1; NP_079491.2
- Function
- Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA.
Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3'.
Acts as a regulator of pre-mRNA splicing by mediating pseudouridylation of pre-mRNAs at locations associated with alternatively spliced regions.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2943 | Hi-Affi™ Recombinant Rabbit Anti-PUS1 Monoclonal Antibody (DS2943AB) | FC, ICC/IF, IHC-P, WB | IgG |
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